Novel Method for ELISA Traces Pipetting System by Air Displacement Pipetting

Abstract:

In the Enzyme-linked immunosorbent assay (ELISA) test, many steps need traces pipetting. The ELISA test results will be different when we use different pipetting ways. Our traces pipetting system is based on the air displacement pipetting principle, comparable to the functioning of hand pipettes. It is applied pressure sensor to realize pressure-based liquid level detection (pLLD) and aspiration monitoring. The monitored system can distinguish the following situations: (1) a correct aspiration; (2) cup empty; (3) tip-blocked; (4) bubbles. Using the air displacement principle into traces pipetting can avoid contamination or dilution by system liquids, and problems with corroded tubing, pumps, etc. It applied pressure sensor to realize pLLD and aspiration monitoring. The results of the real-time monitor module on air displacement pipetting show that the traces pipetting system can agilely distinguish the different liquid pipetting situations. The method of air displacement pipetting offered an effective way for ELISA traces pipetting system.

• Periodical: Applied Mechanics and Materials (Volume 103)
• Pages: 252-256
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.103.252
• Online since: September 2011
• Authors: Lian Qing Zhu, Hong Li, Yun Xiao Na, Yang Kuan Guo, Ming Li Dong
• Keywords: Air Displacement Pipetting, ELISA, Pressure Liquid Level Detection, Traces Pipetting

References

[1] JIAO Kui, ZHANG Shusheng. Analysis and Application of ELISA. Chemical Industry Press, (2004) 84-140.

[2] ZHOU Xiao-ping, LUO Zhan-peng, LI Mei-jun, et al. Establishment and Application of Large Scale ELISA Assay System Validation Method after Manufacturers Calibration. Chinese Journal of Public Health Engineering, Vol. 10, No. 2, (2011) 124-126.

[3] D. Bohrer, P.C. do Nascimento, R. Binotto, J.A.T. Borges da Costa, T. Szlachta. Improvement of precision for pipetting blood serum samples into a graphite furnace. Spectrochimica Acta Part B: Atomic Spectroscopy, Volume 57, Issue 12, ( 2002) 2167-2173.

DOI: 10.1016/s0584-8547(02)00170-2

[4] YE Jieming, DING Guoqing. Confirmation of Pipetting Performances for Fully-Open Automatic Biochemistry Analyzers. Chinese Journal of Medical Instrumentation, Vol. 29, (4), (2005) 277-282.

[5] YANG Lei, LI Guangming, KANG Li, WANG Ning. Signal acquisition for atmospheric pressure transducer based on multi- period measurement. Chinese Journal of Scientific Instrument, Vol. 28, No. 4, (2007) 687-691.

[6] SHU Dongbe. New Liquid Level Transducer. Journal of Transducer Technology. Papers (03), (1995)56-57.

[7] GUO Ruibao, BIAN Wenchao, LUO Qi, etc. Method for Adjusting Analog Values of PTB220 Pressure Sensors. Meteorological Science and Technology, Vo l. 39, No. 2, (2011) 217-221.

[8] ZHONG Xiaoqiang. Design of Liquid Level Controller Based on Single Chip Computer. , Modern Electronics Technique, (2009) 51-58.

[9] YANG Lei, LU Guangjian, FAN Baosong. Signal acquisition and nonlinear correction for atmospheric pressure transducer. Journal of Transducer Technology, Vol. 24, No. 9, (2005) 13-15.