One of tasks of fracture mechanics analysis is to get J resistance curves and fracture toughness of ductile materials. Based on the dimensionless theory, a modified Spb method from the load separation method was proposed. In order to apply load separation method to analysis the fracture toughness of a material from its test data, the testing application J-LSS (J resistance curve-Load Separation Software) has been developed by using visual basic (VB) language and was applied to obtain the fracture toughness values of Cr2Ni2MoV rotor steel and 316L stainless steel. Additionally, the differences between the traditional normalization method and the modified Spb method in analyzing fracture toughness through J-LSS were discussed. The modified Spb method presented in the paper is more available and can be recommended to renew the current material fracture toughness standard test.