The corrosion behavior during friction of refined bio-oil by distillation process was evaluated by high frequency reciprocating test rig. Both scanning electron microscopy (SEM) and energy dispersive spectrometer (EDS) were used to investigate the morphology and active elements contents on the rubbed surface on steel disk. The chemical structures of the oils and the topography and surface roughness of the wear scar were also studied by FTIR and surface roughness tester as well, respectively. The experimental results showed that corrosion behavior of bio-oil was improved after refining since the wear depth was 3.75µm and the worn surface roughness was 0.172µm, while they were 12.5µm and 0.217µm before refining. It was concluded that corrosion degree of worn surface was decreased after the bio-oil was refined, which was ascribed to the decrease of the acidic component content after the refining process.