The Impact of Tetrahydropalmatine on ⁹⁹Tc^m-MIBI Uptake of Human Breast Cancer MCF-7 Cell

Abstract:

Objective: To observe the effects of chemosensitizer on expression of P-glycoprotein (P-gp) in multidrug-resistant (MDR) carcinoma cells and the changes of corresponding ⁹⁹Tc^m-MIBI (referring to methoxy isobutyl isonitrile) uptake kinetics. Methods: Firstly, the immunocytochemistry was used to test the experssion level of P-gp in carcinoma cells; then the γ counter was used to test the uptake rate of radioactive precipitation of ⁹⁹Tc^m-MIBI; and finally to do statistical analysis of the test data. Results: The P-gp expression in MCF-7/S cells was negative, while it was strongly positive in MCF-7/ADM cells, and the P-gp expression in MCF-7/ADM cells reduced significantly after the tetrahydropalmatine (Tet) effect. The uptake rate of MCF-7/S cells reached the maximal value (1.39%) after being cultured with ⁹⁹Tc^m-MIBI for 90 minutes, and then it kept in equilibrium. The ⁹⁹Tc^m-MIBI uptake rate of MCF-7/ADM cells had been maintained at low level, being 0.371% after 90 minutes. The ⁹⁹Tc^m-MIBI uptake rate of MCF-7/S cells was higher than that of MCF-7/ADM cells. After the tetrahydropalmatine and verapamil effects, the ⁹⁹Tc^m-MIBI uptake rate of MCF-7/S cells had no significant change, while the ⁹⁹Tc^m-MIBI uptake rate of MCF-7/ADM cells increased by 1.9 times. Conclusion: Tet can reverse the MDR in MCF-7 cells, and the mechanism can achieve the effect of reversing the drug resistance through adjusting down the expression of P-gp in carcinoma cells.

• Periodical: Applied Mechanics and Materials (Volumes 138-139)
• Pages: 1078-1081
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.138-139.1078
• Online since: November 2011
• Authors: Jun Yun, Ling Wang, Jia Ying Yuan
• Keywords: Human Breast Cancer, Multidrug Resistance (MDR), P-glycoprotein (P-gp), Tetrahydropalmatine

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