Isolation and Characterization of Denitrifying Bacterium Pseudomonas mendocina aHD7 with Anaerobic Ammonium Oxidization

Abstract:

A denitrifying bacterium aHD7 with nitrate removal efficiency of 91.67% (w/w) in 3 days cultured at 30 °C under stationary conditions was isolated from the activated sludge. The strain was characterized by biochemical tests and 16S rDNA sequence analysis, to be belonging to Pseudomonas. Factors on denitrification ability of aHD7, i.e., pH value, carbon source, C/N ratio, nitrogen source and initial nitrate concentration were investigated. Results showed that this strain could either use nitrate or nitrite as the electron acceptor, and the total inorganic nitrogen removal efficiency for sodium nitrite and potassium nitrate were up to 96.70% (w/w) and 95.93% (w/w) respectively. Denitrification was favored when the pH value was 6-9 and C/N ratio varied from 6 to 8, in which the optimal carbon source was ethanol. The strain had anammox activity, of which the average ammonium utilization rate reached up to 4.56 mg/l/d.

• Periodical: Applied Mechanics and Materials (Volumes 178-181)
• Pages: 699-703
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.178-181.699
• Online since: May 2012
• Authors: Ji Bo Xiao, Hui Xia Jiang, Shu Yi Chu
• Keywords: Anammox Activity, Denitrifying Bacteria, Identification, Isolation, Removal Efficiency

References

[1] A.A. Van de Graaf, P. de Bruijn, L.A. Robertson, M.S.M. Jetten and J.G. Kuenen: Water Res. Vol. 41 (1996), pp.1623-1634

[2] U. Van Dongen, S.M.S. Jetten and M.C.M. van Loosdrecht: Water Sci. Technol. Vol. 44 (2001), pp.153-160

[3] W.R.L. Van der Star, W.R. Abma, D. Blommers, J.W. Mulder, T. Tokutomi, M. Strous, C. Picioreanu and M.C.M. Van Loosdrecht: Water Res. Vol. 41 (2007), pp.4149-4163

DOI: 10.1016/j.watres.2007.03.044

[4] B. Kartal, J.G. Kuenen and M.C.M. van Loosdrecht: Science 328 (2010), pp.702-703 .

[5] S. Philips, H.J. Laanbroek and W. Verstraete: Rev. Environ. Sci. Biotechnol. Vol. 1 (2002), pp.115-141

[6] G. Ruiz, D. Jeison and R. Chamy : Water Res. Vol. 37 (2003), pp.1371-1377

[7] T. Khin and A.P. Annachhatre: Biotehnol. Adv. Vol. 22 (2004), pp.519-532

[8] H.W. Sun, Q. Yang, Y.Z. Peng, X.N. Shi, S.Y. WANG and S.J. Zhang : Chin. J. Chem. Eng. Vol. 17 (2009), pp.1027-1031

[9] P. Zheng, F.M. Lin, B.L. Hu and J.S. Chen: J. Environ. Sci. Vol. 16 (2004), pp.13-16

[10] N. Takaya, M.A.B. Catalan-Sakairi, Y. Sakaguchi, I. Kato, Z. Zhou and H. Shoun: Appl. Environ. Microbiol. Vol. 69 (2003), pp.3152-3157

DOI: 10.1128/aem.69.6.3152-3157.2003

[11] J.G. Holt, N.R. Kreig, P.H. Sneath, J.T. Staley and S.T. Williams, Bergey's manual of determinative bacteriology (Williams and Wilkins Publishers, Baltimore 1994).

[12] APHA, AWWA, WEF, Standard methods for the examination of water and wastewater (American Public Health Association, Washington 1998).

[13] O. Modin, K. Fukushi and K. Yamamoto: Water Res. Vol. 41 (2007), pp.2726-2738

[14] J.J. Her and J.S. Huang: Bioresour. Technol. Vol. 54(1995), pp.45-51

[15] B.L. Hu, P. Zheng, J.Y. Li, X.Y. Xu and R.C. Jin: Sci. China Ser. C. Vol. 49 (2006), pp.460-466

[16] E. Broda: Z. Allg. Mikrobiol. Vol. 17(1977), pp.491-493