Arsenite Oxidation Characteristics and Molecular Identification of Arsenic-Oxidizing Bacteria Isolated from Soil

Abstract:

Arsenite oxidation characteristics were studied through domesticated bacteria from soil added arsenic artificially, which came from Dinghu Mountain Natural Reserve of Zhaoqing, Guangdong, China. Two individual bacterial strains were selected as arsenite-oxidizing bacteria by reaction of silver nitrate and detected in community DNA fingerprints generated by PCR coupled with denaturing gradient gel electrophoresis. Physiological, biochemical and arsenite oxidation characteristics of arsenic-oxidizing bacteria were researched. They were gram-negative and rod-shaped bacteria, which were 99% related to Alcaligenes sp.(strain H) and 100% related to Agrobacterium sp. (strain Q) respectively. The arsenic-oxidizing experiment showed that the optimal temperature and pH were 30°Cand 9.0 respectively for both strains. Strain H was an efficient arsenite-oxidizing bacteria. It oxidized As(III) by nearly 100% after 21 hours. Therefore, It was detected as the most perspective strains in this study.

• Periodical: Applied Mechanics and Materials (Volume 188)
• Pages: 313-318
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.188.313
• Online since: June 2012
• Authors: Wei Feng Song, Qi Deng, Li Ying Bin, Wei Wang, Chun Wu
• Keywords: 16SrDNA, Agrobacterium, Alcaligenes, Arsenic, Characteristic, Oxidation

References

[1] Mukhopadhyay,R., Rosen B.P. , Pung L.T. and Silver, S. Microbial arsenic: from geocycles to genes and enzymes. FEMS Microbiol. Rev. 26( 2002) 311.

DOI: 10.1111/j.1574-6976.2002.tb00617.x

[2] Rosen, BP, Biochemistry of arsenic detoxification. FEBS Lett. 529(2002) 529.

[3] Oremland, RS and Stolz, JF, The ecology of arsenic. Science. 300(2003) 939.

[4] Cullen W.R. and Reimer K.J., Arsenic speciation in the environment. Chem. Rev. 89(1989)713.

[5] Rhine, E.D., Garcia D.E., Phelps C.D. and Young, L.Y., Environmental microbes can speciate and cycle arsenic. Environ. Sci. Technol. 39( 2005) 9569.

DOI: 10.1021/es051047t

[6] Routh J, Saraswathy A, Matthew D. C. Arsenicicoccus bolidensis a novel arsenic reducing actinomycete in contaminated sediments near the Adak mine (northern Sweden): Impact on water chemistry. Science of the Total Environment, 379(2007)216–225.

DOI: 10.1016/j.scitotenv.2006.06.027

[7] Green H.H., Isolation and description of a bacterium causing oxidation of arsenite to arsenate in cattle-dipping baths. Rep. Dir. Vet. S. Afr. 6(1918) 593.

[8] Lugtu R.T., Choi S.C. and Oh,Y.S., Arsenite Oxidation by a Facultative Chemolithotrophic Bacterium SDB1 Isolated from Mine Tailing.J. Microbiol.  47(2009) 686.

DOI: 10.1007/s12275-009-0279-3

[9] Chang J.S. , Yoon I.H. , Lee J.H., Kim K.R., An,J., and Kim K.W. , Arsenic detoxification potential of aox genes in arsenite-oxidizing bacteria isolated from natural and constructed wetlands in the Republic of Korea. Environ. Geochem. Hlth. 267( 2010) 95.

DOI: 10.1007/s10653-009-9268-z

[10] Drewniak,L., Matlakowska,R. , Rewerski,B., and Sklodowska, A., Arsenic release from gold mine rocks mediated by the activity of indigenous bacteria. Presented at 18th International Biohydrometallurgy Symposium , Bariloche, Argentina, September 13-17 (2010).

DOI: 10.1016/j.hydromet.2010.02.025

[11] Garcia D.E., Mumford,A., Rhine, E.D., Paschal, A. and Young, L.Y., Novel autotrophic arsenite-oxidizing bacteria isolated from soil and sediments. FEMS Microbiol. Ecol. 66 ( 2008)401.

DOI: 10.1111/j.1574-6941.2008.00569.x

[12] Connon S.A., Koski A.K. , Neal, A.L., Wood, S.A., and Magnuson, T.S., Ecophysiology and geochemistry of microbial arsenic oxidation within a high arsenic, circumneutral hot spring system of the Alvord Desert. FEMS Microbiol. Ecol. 64 (2008)117.

DOI: 10.1111/j.1574-6941.2008.00456.x

[13] Anderson ,G.L., Williams ,J., and Hille ,R.L., The purification and characterization of arsenite oxidase from Alcaligenes faecalis, a molybdenum-containing hydroxylase. J. Biol. Chem. 267 (1992). 23674.

DOI: 10.1016/s0021-9258(18)35891-5

[14] Salmassi T.M., Venkateswaren,K. & Satomi,M., Nealson, K.H., Newman, D.K., and Hering, J.G. Oxidation of arsenite by Agrobacterium albertimagni, AOL15, sp. nov., isolated from hot creek, California. Geomicrobiol. J. 19(2002). 53.

DOI: 10.1080/014904502317246165

[15] Santini J.M., Sly L.I. & Schnagl R.D., and Macy, J.M. A new chemolithoautotrophic arsenite-oxidizing bacterium isolated froma goldmine: Phylogenetic, physiological, and preliminary biochemical studies. Appl. Environ. Microb. 66(2000) 92.

DOI: 10.1128/aem.66.1.92-97.2000

[16] Barra C.M., Cervera M.L., de la Guardia, M., and Santelli, R.E. Atomic fluorescence determination of inorganic arsenic in soils after microwave-assisted distillation. Anal. Chim. Acta. 407(2000) 155.

DOI: 10.1016/s0003-2670(99)00823-5

[17] Coelho N.M.M. and Parrilla,C. High performance liquid chromatography—atomic fluorescence spectrometric determination of arsenic species in beer samples. Anal. Chim. Acta. 482(2003)73.

DOI: 10.1016/s0003-2670(03)00203-4

[18] Dilana D S, Didier L. Microplate screening assay for the detection of arsenite oxidizing and arsenate reducing bacteria. FEMS Microbiology Letters. 237(2004)249-253.

DOI: 10.1111/j.1574-6968.2004.tb09703.x

[19] Buchanan R.E. and Gibbons N.E. Bergey's Manual of Determinative Bacteriology, 8th ed. Beijing, 1984.

[20] In-Ho,Y., Jin-Soo,C. & Ji-Hoon,L., and Kim, K.W. Arsenite oxidation by Alcaligenes sp. strain RS-19 isolated from arsenic-contaminated mines in the Republic of Korea. Environ. Geochem. Health. 31(2009)109.

DOI: 10.1007/s10653-008-9170-0