Determination of Bisphenol A by Fluorescence Immunoassay Based on Quantum Dots as Probe

Abstract:

A sensitive and selectivity fluoroimmunoassay was established for the determination of bisphenol A, on the basis of being labeled quantum dots. Anti-rabbit IgG conjugated by quantum dots were regarded as a probe in this system. The presented method produced a widely linear range from 0.0001 to 100.0 ng mL-1, with detection limit of 0.078 pg mL-1. The method was applied to determine bisphenol A in plastic leach liquor, with the recoveries from 99.8% to 102.5%. The results were similar to those obtained from a high performance liquid chromatography method, while this method is simple, rapid, sensitive and accurate.

• Periodical: Applied Mechanics and Materials (Volumes 190-191)
• Pages: 452-456
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.190-191.452
• Online since: July 2012
• Authors: Xia Zhang, Yu Bo Pang, Yu Yan Song, Shu Hao Wang
• Keywords: Bisphenol A, Fluoroimmunoassay, Quantum Dot (QD)

References

[1] Zhan, M. J.; Yang, X.; Xian, Q. M.; Kong, L. R. Photochemical transformation of bisphenol A promoted by nitrate ions. Bull Environ Contam Toxicol 2006, 76, 105-112.

DOI: 10.1007/s00128-005-0895-5

[2] Sajiki, J.; Hasegawa, Y.; Hashimoto, H. Determination of bisphenol A (BPA) in plasma of hemodialysis patients using three methods: LC/ECD, LC/MS, and ELISA. Toxicology Mechanisms and Methods 2008, 18, 733-738.

DOI: 10.1080/15376510802290900

[3] Brenn-Struckhofovaa, Z.; Cichna-Markl, M. Determination of bisphenol A in wine by sol-gel immunoaffinity chromatography, HPLC and fluorescence detection. Food Additives and Contaminants 2006, 23, 1227-1235.

DOI: 10.1080/02652030600654382

[4] Cobellis, L.; Colacurci, N.; Trabucco, E. Measurement of bisphenol A and bisphenol B levels in human blood sera from healthy and endometriotic women. Biomedical Chromatography 2009, 23, 1186-1190.

DOI: 10.1002/bmc.1241

[5] Chen, Y. P.; Ning, B. A.; Liu, N.; Feng, Y.; Liu, Z.; Liu, X. Y.; Gao, Z. X. A rapid and sensitive fluoroimmunoassay based on quantum dot for the detection of chlorpyrifos residue in drinking water. Journal of Environmental Science and Health Part B 2010, 45, 508-515.

DOI: 10.1080/03601234.2010.493476

[6] Fan, J.; Guo, H. Q.; Liu, G. G.; Peng, P. A. Simple and sensitive fluorimetric method for determination of environmental hormone bisphenol A based on its inhibitory effect on the redox reaction between peroxyl radical and rhodamine 6G. Analytica Chimica Acta 2007, 585, 134-138.

DOI: 10.1016/j.aca.2006.12.026

[7] García-Prieto, A.; Lunar, M. L.; Rubio, S.; Pérez-Bendito, D. Determination of urinary bisphenol A by coacervative microextraction and liquid chromatography–fluorescence detection. Analytica Chimica Acta 2008, 630, 19-27.

DOI: 10.1016/j.aca.2008.09.060

[8] Kuruto-Niwa, R.; Tateoka, Y.; Usuki, Y.; Nozawa, R. Measurement of bisphenol A concentrations in human colostrums. Chemosphere 2007, 66, 1160-1164.

DOI: 10.1016/j.chemosphere.2006.06.073

[9] Bendito, M. D. P.; Bravo, S. R.; Reyes, M. L. L.; Prieto, A. G. Determination of bisphenol A in canned fatty foods by coacervative microextraction, liquid chromatography and fluorimetry. Food Additives and Contaminants 2009, 26, 265-274.

DOI: 10.1080/02652030802368740

[10] Viganò, L.; Benfenati, E.; van Cauwenberge, A.; Eidem, J. K.; Erratico, C.; Goksøyr, A.; Kloas, W.; Maggioni, S.; Mandich, A.; Urbatzka, R. Estrogenicity profile and estrogenic compounds determined in river sediments by chemical analysis, ELISA and yeast assays. Chemosphere 2008, 73, 1078-1089.

DOI: 10.1016/j.chemosphere.2008.07.057

[11] Feng, Y.; Ning, B. A.; Su, P.; Wang, H. Y.; Wang, C. H.; Chen, F. S.; Gao, Z. X. An immunoassay for bisphenol A based on direct hapten conjugation to the polystyrene surface of microtiter plates. Talanta 2009, 80, 803-808.

DOI: 10.1016/j.talanta.2009.07.070

[12] Zhao, M. P.; Li, Y. Z.; Guo, Z. Q.; Zhang, X. X.; Chang, W. B. A new competitive enzyme-linked immunosorbent assay (ELISA) for determination of estrogenic bisphenols. Talanta 2002, 57, 1205-1210.

DOI: 10.1016/s0039-9140(02)00207-2

[13] Kaddar, N.; Bendridi, N.; Harthé, C.; de Ravel, M. R.; Bienvenu, A. L.; Cuilleron, C. Y.; Mappus, E.; Pugeat, M.; Déchaud, H. Development of a radioimmunoassay for the measurement of bisphenol A in biological samples. Analytica Chimica Acta 2009, 645, 1-4.

DOI: 10.1016/j.aca.2009.04.036

[14] Kim, A.; Li, C. R.; Jin, C. F.; Lee, K. W.; Lee, S. H.; Shon, K. J.; Park, N. G.; Kim, D. K.; Kang, S. W.; Shim, Y. B. A sensitive and reliable quantification method for bisphenol A based on modified competitive ELISA method. Chemosphere 2007, 68, 1204-1209.

DOI: 10.1016/j.chemosphere.2007.01.079

[15] Soukka, T.; Paukkunen, J.; Harma, H.; Lonnberg, S.; Lindroos, H.; Lovgren, T. Supersensitivie time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology. Clin Chem. 2001, 47, 1269-1278.

DOI: 10.1093/clinchem/47.7.1269

[16] Chan, W. C. W.; Nie, S. W. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 1998, 281, 2016-(2018).

DOI: 10.1126/science.281.5385.2016

[17] Yang, Y.; Jing, L.; Yu, X.; Yan, D.; Gao, M. Coating aqueous quantum dots with silica via reverse microemulsion method: toward size-controllable and robust fluorescent nanoparticles. Chem Mater 2007, 19, 4123-4128.

DOI: 10.1021/cm070798m

[18] Jana, N. R.; Earhart, C.; Ying J. Y. Synthesis of water-soluble and functionalized nanoparticles by silica coating. Chem Mater 2007, 19, 5074-5082.

DOI: 10.1021/cm071368z

[19] Wang, C.; Ma, Q.; Dou, W. C.; Kanwal, S.; Wang, G. N.; Yuan, P. F.; Su, X. G. Synthesis of aqueous CdTe quantum dots embedded silica nanoparticles and their applications as fluorescence probes. Talanta 2009, 77, 1358-1364.

DOI: 10.1016/j.talanta.2008.09.018

[20] Ding, S. Y.; Chen, J. X.; Jiang, H. Y.; He, J. H.; Shi, W. M.; Zhao, W. S.; Shen, J. Z. Application of quantum dot−antibody conjugates for detection of sulfamethazine residue in chicken muscle tissue. J. Agric. Food Chem. 2006, 54, 6139-6142.

DOI: 10.1021/jf0606961

[21] Tang, D.; Wang, Y. C.; Chang, W. B.; Ci, Y. X.; Guo, Z. Q. Time-resolved fluorescence immunoassay of estradiol in serum. Chin J Anal Chem 1999, 27, 899-903.