Repeated Batch Biotransformation from Isoeugenol to Vanillin by Immobilized Bacillus fusiformis Cells in CSTR Reactor

Li Qing Zhao; Jia Mao Fang; Wei Bin Chen; Xiang Dong Xiao

doi:10.4028/www.scientific.net/AMM.209-211.1170

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 209-211Repeated Batch Biotransformation from Isoeugenol...

Repeated Batch Biotransformation from Isoeugenol to Vanillin by Immobilized Bacillus fusiformis Cells in CSTR Reactor

Abstract:

Bacillus fusiformis CGMCC1347, screened from soil, was immobilized successfully to biotransform isoeugenol to vanillin. The optimal immobilization conditions were 2.5% sodium alginate, 10% cell density and Ca2+ 0.1 mol l-1. The optimal reaction conditions in a 2 L CSTR reactor with immobilized Bacillus fusiformis CGMCC1347 cells were isoeugenol 600 mL, immobilized cells 400 g, pH 3.5 dH2O 400 mL, 37 oC, 200 r min-1 shaking speed and 0.8 vvm air capacity. The average vanillin concentration reached 39.26 g l-1 after 6 times reuse of immobilized cells in 2 L CSTR.

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Periodical:

Applied Mechanics and Materials (Volumes 209-211)

Pages:

1170-1173

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.209-211.1170

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Online since:

October 2012

Authors:

Li Qing Zhao, Jia Mao Fang, Wei Bin Chen, Xiang Dong Xiao

Keywords:

Bacillus fusiformis CGMCC1347, Biotransformation, Cells Immobilization, CSTR

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DOI: 10.4028/www.scientific.net/amr.554-556.1507

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