Design and Simulation of a Novel Micro-Mixer for Detecting Cell Status in Bio-Artificial Liver

Abstract:

Purposes: To improve the mixing effect in micro-scale for detecting liver cells status timely in bioreactor and improving liver cells growth environment to ensure efficacy of the bio-artificial liver support system(BLASS). Methods: Alanine aminotransferase(ALT) was chosen as an indicator to evaluate the status of liver cells by detecting the absorbance of ALT. A double-layer micro-mixer was designed and optimized to achieve efficiently mix between serum and ALT detection reagent. Results: The simulated results show that the optimized size of the micro-mixer is that the junction length L is 25 μm and the confluence width W is 50 μm. In this structure, satisfactory mixing effect can be achieved. Conclusions: This micro-mixer combines the superiority of active and passive mixer to achieve fast, efficient and easy control mixing. It laies the foundation for online monitoring of liver cells and will help to improve the viability of liver cell in the bioreactor.

• Periodical: Applied Mechanics and Materials (Volumes 246-247)
• Pages: 241-245
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.246-247.241
• Online since: December 2012
• Authors: Yue Cao, Chang Zhe Wu, Xiao Lin Huo, Ming Li
• Keywords: ANSYS, Bio-Artificial Liver, Hydrodynamic, Mass Fraction, Micro-Mixer

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