Rapid Discrimination of Bacteria and Bacteriophages by Raman Spectroscopy

Abstract:

An accurate, high sensitivity and rapid identification assay of bacterial and virus is extremely important in areas such as medical diagnosis, biological research, and environmental monitoring. Raman spectra generates from the molecular species often referred to as ‘whole-organism fingerprinting’ being able to give comprehensive quantitative information about the overall biochemical composition of a microbial sample. Rapid discrimination of bacteria and bacteriaphage can be great important for food, environment and clinical diagnosis. Raman spectra of bacteria stains (Salmonella, Acinetobacter baumannii, Klebsiella pneumoniae) and their related bacteriaphages were analyzed. Raman pattern indicated the characteristic peaks and intensity difference between either bacteria nor phages. Barcode analysis, the intensity of 21 majority peaks summarized from the spectral interval 400–1200 nm showed the distinguishable data code for those cells. The present results suggest that both bacteria (micron) and phages (sub micron) can be directly discriminated by their Raman fingerprint and the barcode analysis method may help us transfer the complex spectra into easily and readability data

• Periodical: Applied Mechanics and Materials (Volumes 284-287)
• Pages: 523-527
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.284-287.523
• Online since: January 2013
• Authors: Chi Chang Lin, Chih Hsun Hung, Chia Wen Chang, Li Rong Huang
• Keywords: Bacteria, Bacteria Fingerprint, Bacteriaphages, Raman Spectroscopy

References

[1] C. Prinz, M. Schoniger, R. Rad, I. Becker, E. Keiditsch, S. Wagenpfeil, M. Classen, T. Rosch, W. Schepp and Gerhard, M. Can. Res. 61, 1903-1909(2001).

[2] S.A. Barshick, D.A. Wolf and A.A. Vass, Anal. Chem. 71, 633-641(1999).

[3] H.G.M. Edwards, N.C. Russell, R. Weinstein and D.D. Wynn-Williams, J. Raman Spectrosc. 26, 911-916(1995).

DOI: 10.1002/jrs.1250260843

[4] K. Maquelin, L.P. Choo-Smith, H.P. Endtz, H.A. Bruining and G.J. Puppels, J. Clin. Microbiol. 40, 594-600(2002).

DOI: 10.1128/jcm.40.2.594-600.2002

[5] M.S. Ibelings, K. Maquelin, H.P. Endtz, H.A. Bruining and G.J. Puppels, Clin. Microbiol. Infect. 11, 353-358(2005).

DOI: 10.1111/j.1469-0691.2005.01103.x

[6] J.T. Magee, 1993. Whole-organism fingerprinting. In: Goodfellow, M., O'Donnell, A.G. (Eds. ), Handbook of New Bacterial Systematics. Academic Press, London, p.383– 427.

[7] J. Popp, W. Kiefer and M. Schmitt, Mol. Struct. 661-662, 363-369(2003).

[8] D. Petrov, H. Grötsch and G. Volpe, Analy. Chem. 77, 2564 - 2568 (2005).

[9] L. Jones and R. A. Tripp, Nano Letters. 6, 2630-2636(2006).

[10] L. J. Goeller and M. R Riley, Appl. Spec. 61, 679-685 (2007).

[11] T. Karsshima and M. Yamamoto, Biochem. 44, 10009-10019(2005).

[12] S. D. Moore and Jr., Biochem. 40, 13583-13591(2001).

[13] Z. Alavidze, A. Sulakvelidze, JR., and J. G. Morris, Antimicrob. Agents Chemother. 45, 649-659 (2001).

DOI: 10.1128/aac.45.3.649-659.2001

[14] J. M. Benevides, M. Tsuboi, and G. J. Thomas, Biophy. J. 72, 2748-2762(1997).

[15] E. Strauch, J. A. Hammerl and S. Hertwig, J. Verbr. Lebensm, 2, 138-143(2007).

[16] J. Filik and N. Stone, SPIE, 6853, 685309-1(2008).