The influence mechanism of rolling motion on the flowing and heat transfer characteristics of turbulent flow in typical four rod bundles is investigated with FLUENT code. The flowing and heat transfer characteristics of turbulent flow in rod bundles can be affected by rolling motion. But the flowing similarity of turbulent flow in adiabatic and non-adiabatic can not be affected. If the rolling amplitude is big or if the rolling period is small, the radial additional force can make the parameter profiles and the turbulent flowing and heat transfer change greatly. And the frictional resistance coefficient and heat transfer coefficient can not be solved by the correlations in steady state. In rolling motion, as the pitch to diameter ratio decrease, especially if it is less than 1.1, the flowing and heat transfer of turbulent flow in rolling motion change significantly.