Screening of Microorganisms Capable of Producing Ethanol by Direct Fermentation of D-Xylose

Abstract:

A total of 120 D-xylose fermenting yeast strains were isolated from composition soil samples. 6 strains capable of fermenting D-xylose to produce ethanol were obtained by TTC double medium agar method screening and potassium dichromate oxidation method re-screening. All the 6 strains belong to the genera Candida or Pichia by morphology and physiology identification. Candida spp. strains showed rather high efficiency to produce ethanol from D-xylose than the Pichia spp. strains, of which, strain M-105 exhibited a D-xylose consumption rate of 98.28% and the highest ethanol yield (0.465 g/g), with concentration up to 18.58 g/L under the condition of fermenting 40 g/L of this sugar at 28°C and 100 r/min for 72 hours. An average ethanol concentration of 18.35±0.07 g/L was reached from three batches fermentation of M-105 in shaking flask.

• Periodical: Applied Mechanics and Materials (Volumes 291-294)
• Pages: 230-233
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.291-294.230
• Online since: February 2013
• Authors: Zhi Tang Lu, Sheng Xun Lin, Da Wei Zhang, Huan Dong
• Keywords: D-Xylose, Ethanol, Fermentation, Screening, Yeast

References

[1] C.Z. Wu, L.L. Ma: Biomass Energy Modern Use of Technology (Chemical Industry Press, China 2003). (In Chinese).

[2] J.F. Hong, M.H. Zhang, C. Liu, S.L. Zou and J.C. Wu: Food and Fermentation Industries Vol. 31(1) (2005), pp.114-118. (In Chinese).

[3] A. Margaritis and P. Bajpai: Appl Environ Microbiol Vol. 44 (1982), pp.1039-1041.

[4] R.M. Cadete, M. A. Melo, K.J. Dussán, R. Rodrigues, S.S. Silva, J.E. Zilli, M.J.S. Vital, F.C.O. Gomes, M. Lachance and C.A. Rosa: PLOS ONE Vol. 7(8)(2012), pp.1-11.

DOI: 10.1371/journal.pone.0043135

[5] B.S. Dien, M.A. Cotta, T.W. Jeffries: Appl Microbiol Biotechnol Vol(63). (2003), pp.258-266.

[6] S. Lynda: Biotechnol Lett , Vol. 10(8) (1988), pp.603-608.

[7] E.G. Wene, A.A. Antonopoulos: Biomass Vol. 3(1988), pp.13-19.

[8] H. Tamakawa, S. Ikushima and S. Yoshida: Biosci, Biotech & Biochem Vol. 75 (2011), p.1994- (2000).

[9] T. Tanino, T. Ito and C. Ogino: J Biosci Bioeng. Vol. 114(2) (2012), pp.209-211.

[10] B.Y. Dong, Y.F. Chen, R.X. Yue and D.G. Xiao: Liquor-Making Sci. & Technol. (10) (2008), pp.40-43. (In Chinese).

[11] Y.L. He, Q.L. Hu, X.J. Su and J.Y. Xiong: Journal of Hunan Agriculture University (Natural Sciences) Vol. 37(4) (2011), pp.456-460.

[12] X.Y. Zhang: Fermentation principle (Science Press, China 2005). (In Chinese).

[13] J.C. du Preez: Enzyme and Microbial Technology Vol. 16 (8). (1994), pp.943-955.

[14] M.F. Ma and Y.Z. Zhang: Liquor-Making Sci. & Technol. (2)(2007), pp.93-94. (In Chinese).

[15] J.L. Wang, G. X. Nie, X.L. Cao, J.X. Zhang and P.F. Zhang: Food Res. Develop Vol. 31(7) (2010), pp.1-4. (In Chinese).

[16] C.P. Kurtzman, J.W. Fell and T. Boekhout: The yeasts: a taxonomic study (5th edition). (Elsevier Science, Netherlands 2011).

[17] Y. Lin, J.B. Zhang and Z.F. Xiang: J. South China Univ. Tech. (Natural Science Edition) Vol. 34(5) (2006), pp.33-37. (In Chinese).

[18] J. P. A. Silva, S. I. MussattoI, C. Roberto and J. A. Teixeira: Braz. J. Chem. Eng. Vol. 28(1) (2011) , pp.151-156.