Biological Toxicity of Five Metal Ions on Marine Algea

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Internationally validated methods (ISO standards) for the determination of toxic effects were used in this study to evaluate the toxicity of metals [i.e. Hg, Cu, Zn, Pb and Cd] to a kind of marine algae, namely Chlorella autotrophica. In the test, the no observable effect concentration (NOEC) and the effective concentration for 50% of test algae (EC50) were obtained after 96-h of incubation at 25±1°C, by comparing the growth of the Chlorella autotrophica in the test samples and in the control. Among the 5 metal ions, mercury (Hg) was found to be the most toxic metal in the test (EC50=1.04 mmol/L), while cadmium (Cd) was the least toxic (EC50=151.37 mmol/L). The results from the Chi-square test of each dose-response equations showed that 96h-EC50s calculated were all precise and credible. According to both NOEC and 96h-EC50, the toxic sequence of these metals from high to low on the inhibition of Chlorella autotrophica’s growth was Hg2+, Cu2+, Zn2+, Pb2+ and Cd2+.

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17-20

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February 2013

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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