Research and Application on the Effect of Magnaporthe grisea Toxin on Rice

Abstract:

Knowledge remains limited about whether the optimum culture condition of getting the most virulent M.oryzae toxins can be found and applied for resistant seed selection. The inhibition of seed germination and the growth of embryo and radicle in rice treated by the Magnaporthe grisea toxin that got from different culture conditions were studied. The result suggested that the M.oryzae toxin had great inhibitory effect on seed germination and growth of embryo and radicle in rice. The M.oryzae toxin cultivated on 23 °C, pH 8.0 for 20d has strongest inhibition on seed germination of the hybrid rice variety of GangYou725, the inhibition rate approach 70%; Being cultivated at the same pH and 23°C～28°C for approximate 10d, it is easy to get the M.oryzae toxin for high growth inhibition of embryo and radicle. It can reduce the workload and increase accuracy to use M.oryzae toxin to identify blast resistances of rice and select resistant materials.

• Periodical: Applied Mechanics and Materials (Volumes 295-298)
• Pages: 2333-2337
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.295-298.2333
• Online since: February 2013
• Authors: Yun Gao Hu*, Guo Tao Yang, Chong Hua Xie, Ling Zhang, Yong Jun Chen
• Keywords: Identify and Select, M.oryzae Toxin, Rate of Germination Inhibition, Rate of Growth Inhibition

References

[1] Li Y., Nie Y.Y., Cai Y.H., et al. Acta Agriculturae Jiangxi: Research Progress in Rice Blast Resistant Genes in Rice and Their Molecular Marker Assisted Selection Breeding, Vol. 23, No. 9(2011), pp.72-75.

[2] Yang G.T., Zhang L. and Zhu W. k. Hubei Agricultural Sciences: Effect of Crude Toxin from the Phyricularis Oryzae. cav on the Callus of Rice, Vol. 47, No. 8 (2008), pp.855-858.

[3] Yang Q.Y., Xu X.P. and Feng S.J., et al. Acta Phytopathologica Sinica: Study on the Blast Resistance of transgenic Rice Plants with Tow Antifungal Protein Genes, Vol. 33, No. 2(2003), pp.162-166.

[4] Yang Q.Z., Lin F., Feng S.J., et al. Scientia Agricultura Sinica : Recent Progress on Molecular Mapping and Cloning of Blast Resistance Genes in Rice(Oryza sativa L. ), Vol. 42, No. 5(2009), pp.1601-1615.

[5] Xie H.J., Wang J.L., and Chen G.H. 2006. Crop Research: Advances in Rice Blast Resistance Breeding. Changsha: Hunan Agricultural University, Vol. 5(2006), pp.417-421.

[6] Zheng Z., Chen Y. Q, Zhang J. F, et al. Molecular Plant Breeding: Mapping, Cloning of Rice Blast Resistance Genes and Their Application, Vol. 7, No. 2(2009), pp.385-392.

[7] Ren J.S., Xiao P.C., Chen Y., et al. 2009. Scientia Agricultura Sinica: Analysis on Blast Resistance Phenotypes and Resistance Gene Analog Polymorphism of Rice Varieties, Vol. 42, No. 1(2009), pp.1-9.

[8] Ye Y, Fan J. H, Meng Y. N, et al. Journal of Yunnan Agricultural University: Changes of CAT and PPO Activities and MDA Content of Rice during Induction by Rice Blast Crude Toxin, Vol. 23, No. 6(2008), pp.775-780.

[9] Pan Z. C, Chen J.B., Fan J.H., et al. Journal of Yunnan Agricultural University: Activities of Defense-related Enzymes Induced by Crude Toxin from Magnaporthe grisea, Vol. 23, No. 2(2008), pp.162-166.

[10] Chen G., Zhong M., Hou Y.Z., et al. Seed: Determination of Pathogenicity of Rice Blast Crude Toxin and the Influence of Physiological and Biochemical in Rice Seedling, Vol. 25, No. 5(2006), pp.20-24.

[11] Liu W.P., Liu L.Y., Lv X.B., et al. Heilongjiang Agricultural Science: Preparation of Crude Toxin of Pyricularia Oryzae and Determination of its Disease Producing Ability, Vol. 6 (1997), pp.16-17.

[12] Luo M.J. and Zhu T.H. Journal of Sichuan Forestry Science and Technology: Phytotoxin in Plant Pathogen, Vol. 3, No. 22(2001), pp.1-5.