Qualitative and Quantitative Analysis of Flavonoids in Humulus scandens (Lour.)Merr. by UPLC-TQD and HPLC

Abstract:

To qualify and quantify the flavonoids existing in Humulus scandens (Lour.)Merr., a Ultra performance liquid chromatography triple-quadrupole tandem mass spectrometer (UPLC-TQD) method was applied to preliminarily analyze the flavonoids exiting in Humulus. And a High performance liquid chromatography (HPLC) method was used to analyze the flavonoids existing in Humulus. Flavonoids in methanol extracts of Humulus were cleaned up by a C18 cartridge, separated by a PLASMA ODS column(5μm,250×4.6mm), with a gradient elution of methanol and 0.1% formic acid at a flow rate of 1 mL•min^-1 . Analytes were detected with ultraviolet detector (UV) at 280nm. Flavonoids, including Epicatechin, Luteolin-7-O-Glucoside, Vitexin or Apigenin-7-glucoside, Morin hydrate or Quercetin dehydrate and Luteolin were found in Humulus according to the UPLC-TQD. Results of HPLC analysis showed the linear ranges of 11 flavonoids were 0.1mg•L^-1-20mg•L^-1 with the correlation coefficient (r) all above 0.9909. Limits of detection (signal/ noise=3) was 100^{Superscript text} μg•L^-1. The average recoveries of all the compounds spiked at four levels of 200μg•L^-1, 500μg•L^-1, 1 mg•L^-1 and 1.5 mg•L^-1 were in the ranges of 70.8%-112.1%, 74.6%-116.6%, 73.1%-110.6%, 73.7%-99.9%, with the corresponding relative standard deviation (RSD, n=6) of 2.5%-5.8%, 0.8%-4.3%, 0.8%-3.7%, 1.7%-3.8%, respectively. This method ,which was proved to be precise, sensitive and reliable, could be applied to analyze flavonoids in leaves.

• Periodical: Applied Mechanics and Materials (Volumes 295-298)
• Pages: 354-359
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.295-298.354
• Online since: February 2013
• Authors: Xiao Wei Wang*, Zhen Hua Liu, Rui Xiang Yu, De Hua Guo, Si Ming Zhu, Cui Hua Xia, Zhuo Ping Ding
• Keywords: Application, Flavonoid, High Performance Liquid Chromatography (HPLC), Humulus Scandens (Lour.)Merr., UPLC

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