For Libraries For Publication Open Access Downloads About Us Contact Us
Paper Titles
Evaluation of Chlorine for Inactivation of Bioterrorism Agents in Drinking Water
p.599
Dilemma and Countermeasures of County Governance in the Context of the Environmental Crisis
p.604
Adsorption of Metal Ions and Organic Ions onto Human Scalp Hair
p.613
Characterization and Health Risk Assessment of PM10-Bound PAHs in Urban Air of Shenyang, China
p.617
A Highly Sensitive Fluorescence Immunoassay for the Determination of Diethylstilbestrol
p.622
Offshore Oil Spill Incidents in Brazil
p.626
Characterization of Antibiotic Resistance E. Coli and Antibiotic Resistance Genes in Aquatic Environment of Taihu Lake, China
p.630
Researches on the Forewarning Indicator System of Groundwater Environmental Health
p.635
Effects of Phthalic Acid Esters on the Ecological Environment and Human Health
p.640

A Highly Sensitive Fluorescence Immunoassay for the Determination of Diethylstilbestrol

Article Preview

Abstract:

A highly sensitive direct competitive fluorescence immunoassay (FIA) was developed for the determination of diethylstilbestrol (DES), based on the antibody coating format using biotin-streptavidin amplification system. In the assay, DES competed with biotinylated goat anti-rabbit IgG for the immobilized antibody, then the streptavidin-FITC was added to link to the biotinylated goat anti-rabbit IgG as a tracer. Under optimal conditions, the assay provided a wide analytical range of 0.001-10000 ng/mL with a detection limit of 2.91×10-4 ng/mL (R2=0.9943). This method was then applied to the determination of DES in chicken with satisfactory results

You might also be interested in these eBooks
Progress in Environmental Protection and Processing of Resource View Preview

Info:

Periodical:

Applied Mechanics and Materials (Volumes 295-298)

Pages:

622-625

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.295-298.622

Citation:

Cite this paper

Online since:

February 2013

Authors:

Xin Yi Wang, Wen Dong, Wei Li Xue, Shu Hao Wang

Keywords:

Antibody Coating Format, Diethylstilbestrol, Direct Competitive Fluorescence Immunoassay

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2013 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] D.M. Fry: Environ Health Perspect Vol. 103 Suppl 7 (1995), p.165.

Google Scholar

[2] C.M. Markey, B.S. Rubin, A.M. Soto and C. Sonnenschein: J. Steroid Biochem. Mol. Biol. Vol. 83 (2003), p.235.

Google Scholar

[3] R.J. Golden, K.L. Noller, L. Titus-Ernstoff, R.H. Kaufman, R. Mittendorf, R. Stillman and E.A. Reese: Crit. Rev. Toxicol. Vol. 28 (1998), p.109.

DOI: 10.1080/10408449891344191

Google Scholar

[4] T. Goloubkova, M.F. Ribeiro, L.P. Rodrigues, A.L. Cecconello and P.M. Spritzer: Arch Toxicol Vol. 74 (2000), p.92.

Google Scholar

[5] P. Tong, Y. Kasuga and C.S. Khoo: J. Food Compos. Anal. Vol. 19 (2006), p.150.

Google Scholar

[6] M.J. Gómez, A. Agüera, M. Mezcua, J. Hurtado, F. Mocholí and A.R. Fernández-Alba: Talanta Vol. 73 (2007), p.314.

DOI: 10.1016/j.talanta.2007.03.053

Google Scholar

[7] L. Wang, P. Yang, Y. Li and C. Zhu: Talanta Vol. 70 (2006), p.219.

Google Scholar

[8] R.S. Zhao, X. Wang and J.P. Yuan: J. Sep. Sci. Vol. 33 (2010), p.1652.

Google Scholar

[9] K. Hosoda, T. Furuta, A. Yokokawa, K. Ogura, A. Hiratsuka and K. Ishii: Drug Metab. Dispos. Vol. 36 (2008), p.1485.

Google Scholar

© 2025 Trans Tech Publications Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies. For open access content, terms of the Creative Commons licensing CC-BY are applied.
Scientific.Net is a registered trademark of Trans Tech Publications Ltd.