Mechanism of Hyaluronic Acid Protect Rabbit Chondrocyte Apoptosis Induced by NO

Abstract:

AIM: To investigate the mechanism of hyaluronic acid on rabbit chondrocyte apoptosis in vitro induced by NO. METHODS: We cultured rabbit chondrocytes as normal group and added SNP after cultured 24 h as model group. Treated group was added HA. We used cell culture experiment. We tested the activity of mitochondria though MTT. The flow cytometry detected mitochondrial membrane potential, the percentage of apoptosis and intracellular free calcium concentration.RESULTS: HA could elevate the active of chondrocyte mitochondria and MMP; it could decrease the rate of chondrocyte apoptosis and intracellular free calcium concentration.CONCLUSION: HA can inhibit the lowering of the MMP in chondrocyte, which has a stable role on MMP and inhibit apoptosis occurred. This effect may be related to inhibiting of intracellular calcium overload chondrocytes.

• Periodical: Applied Mechanics and Materials (Volumes 295-298)
• Pages: 78-81
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.295-298.78
• Online since: February 2013
• Authors: Hua Liu*, Hua Guang Li, Su Liu
• Keywords: Calcium, Hyaluronic Acid, Mitochondria Membrane Potential, NO, Rabbit Chondrocyte

References

[1] Kimura T. Progress of research for osteoarthritis, An overview of the recent knowledge on osteoarthritis: pathogenesis, evaluation and therapies. Clin Calcium, 2009, 19(11): 1565.

[2] Julovi SM, Ito H, Nishitani K, et al. Hyaluronan inhibits matrix metalloproteinase-13 in human arthritic chondrocytes via CD44 and P38. J Orthop Res, 2011, 29(2): 258-64.

DOI: 10.1002/jor.21216

[3] Hashimoto S, Ochs RL, Komiya S, et al. Linkage of chondrocyte apoptosis and cartilage degradation in human osteoarthritis. Arthritis Rheum, 1998, 41(9): 1632-8.

DOI: 10.1002/1529-0131(199809)41:9<1632::aid-art14>3.0.co;2-a

[4] Jin Dadi, Sun Wei, Wang Jixing, et al. Potential clinical evaluation of ni tric oxide synthase inhibitor for th e treatment of osteoarthritis. Chinese Journal of Orthopaedics, 2002, 22(6): 367. In Chinese.

[5] Blanco FJ, Ochs RL, Schwarz H, et al. Chondrocyte apoptosis induce by nitric oxide. Am J Pathol, 1995, 146(1): 75.

[6] Hosono K, Nishida Y, Knudson W, et al. Hyaluronan Oligosaccharides Inhibit Tumorigenicity of Osteosarcoma Cell Lines MG-63 and LM-8 in Vitro and in Vivo via Perturbation of Hyaluronan-Rich Pericellular Matrix of the Cells. Pathology, 2007, 171(1): 274-86.

DOI: 10.2353/ajpath.2007.060828

[7] Brun P, Panfilo D, Gordini D, et al. The effect of hyaluronan on CD44-mediated survival of normal and hydroxyl radical-damaged chondrocytes. Osteoarthritis Cartilage, 2003, 11(3): 208-16.

DOI: 10.1016/s1063-4584(02)00352-7

[8] Susin SA, Zamzami N, Kroemer G. Mitochondria as regulators of apoptosis: doubt no more. Biochim Biophys Acta, 1998, 1366(1-2): 151-65.

DOI: 10.1016/s0005-2728(98)00110-8

[9] Shuai Zhang, Jun Cui, Ping-Ping Shen. The Regulation of Calcium Signaling in Apoptosis. Chinese Journal of Cell Biology, 2007, 29: 785-90. In Chinese.

[10] Yanagida E, Shoji S, Hirayama Y, et al. Functional expression of Ca2+signaling pathways in mouse embryonic stem cells. Cell Calcium, 2004; 36(2): 135-46.

DOI: 10.1016/j.ceca.2004.01.022