Nitrite Stress on Density of Hemocyte and Phenoloxidase Activity in Hemolymph of Charybdis japonica

Abstract:

In order to discuss the effects of nitrite on the density of hemocyte (DHC) and phenoloxidase (PO) activity in hemolymph of Charybdis japonica, the crabs were exposed to six nitrite-N groups (0, 2.0, 4.0, 6.0, 8.0 and 10.0 mg L-1 ambient nitrite-N concentrations). The DHC and PO activity was examined on days 1, 3, 5, 10 and 15 following the stress. Results showed that the two indexes appeared to have a decline trend after an initial ascent. After 1 day treatment, the DHC of five treatments were higher than that of the control group respectively, while the PO activity of 8.0 and 10.0 mg L-1 nitrite-N exposed group decreased compared with control. With time elapsed, the DHC of lower concentration nitrite-N exposed group increased faster, while the PO activity of higher concentration nitrite-N exposed group rised faster than other treatments. After 15-day stress, all parameters tested were lower than those of control group barring the DHC of 2.0 and 4.0 mg L-1 nitrite-N exposed group and the PO activity of 2.0, 4.0, and 6.0 mg L-1 nitrite-N exposed group.

• Periodical: Applied Mechanics and Materials (Volumes 295-298)
• Pages: 82-86
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.295-298.82
• Online since: February 2013
• Authors: Xing Hong Xu, Li Zhou, Yan Qing Liu, Bin Lun Yan
• Keywords: Charybdis Japonica, Density of Hemocyte, Nitrite Stress, Phenoloxid Activity

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