Cloning and Expression of β-Glucosidase Gene from Bacillus pumilus

Lin Li; Qing Long Ji; Jian Feng Hao; Lin Yuan

doi:10.4028/www.scientific.net/AMM.300-301.1271

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 300-301Cloning and Expression of β-Glucosidase Gene from...

Cloning and Expression of β-Glucosidase Gene from Bacillus pumilus

Abstract:

Strain Bacillus pumilus AC-6 was separated from the soil, which can produce β-glucosidase. According to the β-glucosidase gene of strain SAFR-032, primers were designed to clone the β-glucosidase gene from AC-6. The sequence of β-glucosidase gene from AC-6 is 1437 bp and encode 478 amino acids. Compared with β-glucosidase gene reported in GenBank, the similarity of the gene sequence is 98%. We cloned β-glucosidase gene into the vector pGEX-4T-1 and transferred it into the competent E. coli DH5α and BL21（DE3）for expression.

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Periodical:

Applied Mechanics and Materials (Volumes 300-301)

Pages:

1271-1274

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.300-301.1271

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Online since:

February 2013

Authors:

Lin Li, Qing Long Ji, Jian Feng Hao, Lin Yuan

Keywords:

Bacillus pumilus, Gene Cloning, Protein Expression, ß-Glucosidase

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