Paper Titles

Development of a CCD-Based Optical Computed Tomography Scanner Used in 3D Gel Dosimetry
p.1632

Finite Deformation Analysis and Numerical Simulation of Arterial Wall
p.1636

The Research of Applying Parallel Streaming Technology on Developing Cloud Computing Platform for Delivering Medical Images
p.1640

The Illumination of a Electrical Non-Fiberoptic Endoscope of Surgery
p.1645

Cell Separation Through Ascending and Descending Curvilinear Microchannels
p.1649

The Influence of Suture Density on Bioprosthetic Heart Valve
p.1654

Computer Simulation for New Theoretical Model Constructed with Tuberculosis
p.1658

Color Image Enhancement Methods Based on Matlab
p.1664

Design and Implementation of Seismic Data Digital Filter
p.1669

HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 300-301Cell Separation Through Ascending and Descending...

Cell Separation Through Ascending and Descending Curvilinear Microchannels

Article Preview

Abstract:

Separation of rare cells such as fetal cells from blood has potential importance in disease investigation and prevention. In this paper we report a new method of cancer cells separation from patient’s blood by inertial focusing technique. A design and simulation of ascending and descending curvilinear microchannels for separation of particles resembling cancer cells have been presented. Computational fluid dynamics (CFD) design and simulation of ascending and descending microchannels is used for cell separation. The simulation was carried out in two stages including focusing and separation. The ascending curvilinear channel design demonstrated favorable focusing and separation. Separation with 100% purity and efficiency of the unwanted particle was achieved at Reynolds number (Re) = 8.50 and velocity 0.105m/s. Reynolds number 9.25 and 10.06 with corresponding velocities 0.115 m/s and 0.125 m/s were also investigated for cell seperation. In case of descending curvilinear channel, cell separation was not good. Considering cancer cells size about 15 µm, our proposed ascending microchannel is a good candidate for cancer cells separation from blood.

You might also be interested in these eBooks

Mechatronics and Applied Mechanics II

Info:

Periodical:

Applied Mechanics and Materials (Volumes 300-301)

Pages:

1649-1653

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.300-301.1649

Citation:

Cite this paper

Online since:

February 2013

Authors:

W.A.H.S.S. Wewala, Jafar Khan Kasi, Ajab Khan Kasi, Nitin Afzulpurkar

Keywords:

Ascending, Cancer Cell, Computational Fluid Dynamics (CFD), Descending, Micro-Channel, Micro-Fluidics, Simulation

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2013 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] D. D. Carlo, Inertial microfluidics, Lab. Chip 9(2009)3038-3046.

[2] A. Higuchi, S. Yamamiya, B.O. Yoon, M. Sakurai, M. Hara, Peripheral blood cell separation through surface-modified polyurethane membranes, J. Biomed. Mater . Res. A 68 (2003) 34- 42.

DOI: 10.1002/jbm.a.20005

[3] M. Hasan, A. K. Kasi, J. K. Kasi, N. Afzulpurkar, Anodic aluminum oxide (AAO) to AAO bonding and their application for fabrication of 3D microchannel, Nanosci. Nanotech. Lett. 4 (2012) 569- 573.

DOI: 10.1166/nnl.2012.1354

[4] M. Hasan, A. K. Kasi, J. K. Kasi, N. Afzulpurkar, S. Porntheeraphat, W. Sripumkhai, Fabrication of thinner anodic aluminum oxide based microchannels, Adv. Mater. Res. 550-553 (2012) 2046-(2050).

DOI: 10.4028/www.scientific.net/amr.550-553.2046

[5] A. K. Kasi, N. Afzulpurkar, J. K. Kasi, A. Tuantranont, P. Dulyaseree, Utilization of cracks to fabricate anodic aluminum oxide nanoporous tubular and rectangular membrane. J. Vac. Sci. Technol., B, 29 (2011) D1071-D1077.

DOI: 10.1116/1.3604943

[6] J. K. Kasi, A. K. Kasi, N. Afzulpurkar, M. Hasan, S. Pratontep, A. Poyai. Fabrication of three dimensional AAO structures, Nanosci. Nanotech. Lett. 4 (2012) 537-543.

DOI: 10.1166/nnl.2012.1353

[7] A. K. Kasi, J. K. Kasi, N. Afzulpurkar, M. Hasan, Bending, Branching of anodic aluminum oxide nanochannels and their applications, J. Vac. Sci. Technol., B 30 (2012) 031805.

DOI: 10.1116/1.4711246

[8] J. Seo, L. Meng, A. Kole, Membrane-free microfiltration by asymmetric inertial migration, Appl. Phys. Lett. 91(2007) 033901(1-3).

DOI: 10.1063/1.2756272

[9] K. J. Humphry, P. M. Kulkarni, D. A. Weitz, J. F. Morris, H. A. Stone, Axial and lateral particle ordering in finite Reynolds number channel flows, Phys. Fluids. 22 (2010) 081703.

DOI: 10.1063/1.3478311

[10] D. R. Gossett, D. D. Carlo, Particle focusing mechanisms in curving confined flows, Anal. Chem. 81(2009) 8459- 8465.

DOI: 10.1021/ac901306y

[11] M. Radisic, R. K. Iyer, S. K Murthy, Micro- and nanotechnology in cell separation, Int. J. Nanomedicine. 1(2006) 3-14.

[12] A. A. S. Bhagat, H. Bow, H. W. Hou, S. J. Tan, J. Han and C. T. Lim, Microfluidics for cell separation, Med. Biol. Eng. Comput. 48 (2010) 999-1014.

DOI: 10.1007/s11517-010-0611-4

[13] C. V. Durgadas, C.P. Sharma, K. Sreenivasan, Fluorescent and super paramagnetic hybrid quantum clusters for magnetic separation and imaging of cancer cells from blood, Nanoscale 3(2011) 4780- 4787.

DOI: 10.1039/c1nr10900f

[15] W. A. H. S. S Wewala, N. Afzulpurkar , J. K. Kasi, A. K. Kasi, A. Poyai, D. W. Bodhale, Design and simulation of ascending curvilinear micro channel for cancer cell separation from blood, Adv. Res. Mater. 557-559 (2012) 2361-2366.

DOI: 10.4028/www.scientific.net/amr.557-559.2361

[16] A. A. S. Bhagat, S. S. Kuntaegowdanahalli and I. Papautsky, Continuous particle separation in spiral microchannels using dean flows and differential migration, Lab Chip, 8(2008), 1906-(1914).

DOI: 10.1039/b807107a

[17] J. Xuan, M. K. H. Leung, D. Y. C. Leung, M. Ni, Density-induced asymmetric pair of Dean vortices and its effects on mass transfer in a curved microchannel with two-layer laminar stream, Chem. Eng. J. 171 (2011) 216-223.

DOI: 10.1016/j.cej.2011.01.011

[18] K. Nilpueng, S. Wongwises, Flow pattern and pressure drop of vertical upward gas–liquid flow in sinusoidal wavy channels, Exp. Therm. Fluid Sci. 30 (2006) 523-534.

DOI: 10.1016/j.expthermflusci.2005.10.004

[19] J. M. MacInnes, J. Ortiz-Osorio, P. J. Jordan, G. H. Priestman, R.W.K. Allen, Experimental demonstration of rotating spiral microchannel distillation, Chem. Eng. J. 159 (2010) 159-169.

DOI: 10.1016/j.cej.2010.02.030

[20] N. Ali, M Sajid, Z. Abbas, T. Javed, Non-Newtonian fluid flow induced by peristaltic waves in a curved channel, Eur. J. Mech. B. Fluids, 29(2010) 387-394.

DOI: 10.1016/j.euromechflu.2010.04.002

[21] Z. Che, T. N. Wong, N. T Nguyen, An analytical model for a liquid plug moving in curved micro channels , Int. J. Heat. Mass. Trans. 53 (2010) 1977-(1985).

DOI: 10.1016/j.ijheatmasstransfer.2009.12.058

[22] J. P. Matas, V. Glezer, E. Guazzelli, J. F. Morris, Trains of particles in finite-Reynolds-number pipe flow, Phys. Fluids. 16 (2004) 4192- 4195.

DOI: 10.1063/1.1791460

[23] S. Ookawara, D. Street, K. Ogawa, Numerical study on development of particle concentration profiles in a curved microchannel, Chem. Eng. Sci. 61(2006) 3714- 3724.

DOI: 10.1016/j.ces.2006.01.016

[24] D. D. Carlo, J. F. Edd, D. Irimia, R. G. Tompkins, M. Toner, Equilibrium separation and filtration of particles using differential inertial focusing, Anal. Chem. 80(2008) 2204- 2211.

DOI: 10.1021/ac702283m