Optimization of Liquid Culture Conditions of Methamidophos Degrading Enzyme from Staphylococcus rosehbach

Abstract:

The nutritional parameters of methamidophos degrading enzyme, which is isolated from soil from Daqing and identified as Staphylococcus Rosehbach, were studied by response surface methodology (RSM). Glucose, peptone and culture time were analyzed by Box-Behnken design for optimizing their concentrations in the medium and the effect of their mutual interaction on methamidophos degrading enzyme activity. Glucose and soybean protein were found in influencing the methamidophos degrading enzyme activity highly significant. Glucose and soybean protein also showed an effect on the methamidophos degrading enzyme activity in interaction with other variables. The optima values of the parameters obtained through RSM, 0.31% glucose, 0.09% peptone and culture time 72h were applied. Actual value of verifiable experimentation was that the degradation ratio of methamidophos degrading enzyme to methamidophos was 39.8% at the optima conditions.

• Periodical: Applied Mechanics and Materials (Volumes 303-306)
• Pages: 2683-2686
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.303-306.2683
• Online since: February 2013
• Authors: Na Zhang, Qing Qi Guo, Xiaofei Liu, Kun Song, Shuang Wang, Mei Xing Wang, Shuang Li, Tong Yu
• Keywords: Methamidophos Degrading Enzyme, Response Surface Methodology (RSM), Staphylococcus Rosehbach

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