Modal Analysis of Vibrating Diaphragm in the Design of Ultrasonication System Based on ANSYS

Abstract:

An ultrasonication system for cells disruption is developed to extract nucleic acid from cells. The structure and operating principle of the ultrasonication system are explained, after which the key problems are stated. Based on finite element modal analysis, the top-ten natural frequencies of the round-shaped vibrating diaphragm are found having a character of stepped distribution, which can be divided into three grades. To optimize the vibration performance of vibrating diaphragm, simulation based on ANSYS is performed, the results of which show that medium grade frequency decreases with the increase of diameter while increases linearly with the increase of thickness.

• Periodical: Applied Mechanics and Materials (Volumes 303-306)
• Pages: 2784-2789
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.303-306.2784
• Online since: February 2013
• Authors: Long Xue Qiao, Yao Hua Du, Feng Chen, Zhi Cheng, Chao Li, Biao Gu, Chen Yu Li, Hao Duan, Tai Hu Wu
• Keywords: ANSYS, Cells Disruption, Modal Analysis, Natural Frequency, Ultrasonication System, Vibrating Diaphragm

References

[1] P. Belgrader, M. Okuzumi, F. Pourahmadi, D.A. Borkholder and M.A. Northrup. A microfluidic cartridge to prepare spores for PCR analysis. Biosensors & Bioelectronics, Vol. 14 (2000), pp.849-852.

DOI: 10.1016/s0956-5663(99)00060-3

[2] S.O. Rogers and A.J. Bendich. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Molecular Biology, Vol. 5 (1985), pp.69-76.

DOI: 10.1007/bf00020088

[3] J.C. Ensign and R.S. Wolfe. Lysis of bacterial cell walls by an enzyme isolated from a myxobacter. Journal of Bacteriology, Vol. 90 (1965), pp.395-402.

DOI: 10.1128/jb.90.2.395-402.1965

[4] O. Salazar and J.A. Asenjo. Enzymatic lysis of microbial cells. Biotechnology Letters, Vol. 29 (2007), pp.985-994.

DOI: 10.1007/s10529-007-9345-2

[5] I.S. Novella, C. Fargues, G. Grevillot. Improvement of the extraction of penicillin acylase from Escherichia coli cells by a combined use of chemical methods. Biotechnology and Bioengineering, Vol. 44 (1994), pp.379-382.

DOI: 10.1002/bit.260440316

[6] R.J. Falconer, B.K.O. Neill, A.P.J. Middelberg. Chemical treatment of Escherichia coli: Extraction of intracellular protein from uninduced cells. Biotechnology and Bioengineering, Vol. 53 (1997), pp.453-458.

DOI: 10.1002/(sici)1097-0290(19970305)53:5<453::aid-bit2>3.0.co;2-g

[7] M.T. Taylor, P. Belgrader, B.J. Furman, F. Pourahmadi, G.T.A. Kovacs and M.A. Northrup. Lysing bacterial spores by sonication through a flexible interface in a microfluidic system. Analytical Chemistry, Vol. 73 (2001), pp.492-496.

DOI: 10.1021/ac000779v

[8] E.M. Fykse, J.S. Olsen, G. Skogan. Application of sonication to release DNA from bacillus cereus for quantitative detection by real-time PCR. Journal of Microbiological Methods, Vol. 55 (2003), pp.1-10.

DOI: 10.1016/s0167-7012(03)00091-5

[9] M.P. Zhong, X.H. Pan and Y.D. Zhang. Design of ultrasonic machining device for thin-wall cylinder of air compressor. Journal of Zhejiang University (Engineering Science), Vol. 42 (2008), pp.1042-1045.

[10] S. Reh, J.D. Beley, S. Mukhherjee and E.H. Khor. Probabilistic finite element analysis using ANSYS. Structural Safety, Vol. 28 (2006), pp.17-43.

DOI: 10.1016/j.strusafe.2005.03.010

[11] W. Rust, K. Schweizerhof. Finite element limit load analysis of thin-walled structures by ANSYS (implicit), LS-DYNA (explicit) and in combination. Thin-Walled Structures, Vol. 41 (2003), pp.227-244.

DOI: 10.1016/s0263-8231(02)00089-7