Automated Extraction of Plant Viral RNA Using the Nucleic Acid Instrument Based on Magnetic Nanobeads

Abstract:

In this paper, an automated nucleic acid extraction instrument based on magnetic nanobeads is described. Also, a prototype is designed and constructed. Some plant viral RNA extraction experiments were done using the platform. And the experimental results demonstrated that the instrument could be successfully used for extraction of plant viral RNA，and the extracted RNA was successfully used in an automated PCR assay for the detection of plant virus. The Ct values of CGMMV RNA, LSV RNA and ArMV RNA at the initial concentration were 12.51, 17.81 and 23.48.

• Periodical: Applied Mechanics and Materials (Volumes 303-306)
• Pages: 646-649
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.303-306.646
• Online since: February 2013
• Authors: Yong Jie Dou, Xiao Li Zhao, Jian Sheng Xu, Cong Liang Deng, Leng Nie, Yang Yu, Ming Wang, Tao Song
• Keywords: Magnetic Bead Separation, Magnetic Nanobeads, Nucleic Acid Extraction, Plant Viral RNA

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