Sensitive Determination of Bioactive Crocin Analogues in Gardenia jasminoides Ellis Using SPE Prior to HPLC Analysis

Xiao Ling Zhang; Hui Juan Yu

doi:10.4028/www.scientific.net/AMM.303-306.654

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 303-306Sensitive Determination of Bioactive Crocin...

Sensitive Determination of Bioactive Crocin Analogues in Gardenia jasminoides Ellis Using SPE Prior to HPLC Analysis

Abstract:

In this study, the feasibility of single-walled carbon nanotubes (SWCNTs) as solid-phase extraction (SPE) adsorbent for the determination of bioactive crocin analogues in the traditional Chinese Medicine (TCM) of Gardenia Jasminoides Ellis prior to HPLC analysis was studied. Various experimental factors influencing SPE efficiency and HPLC conditions were studied. By using SWCNTs as the SPE adsorbent, the detection limit of 1.19-2.21 fmol (at S/N of 3) were achieved with the preconcentration efficiency of more than 120-folds, indicating this proposed method was very practical for increasing the sensitivity prior to HPLC analysis, and it may provides useful clue for the efficient extraction for the bioactive compounds in TCMs.

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Applied Mechanics and Materials (Volumes 303-306)

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654-658

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https://doi.org/10.4028/www.scientific.net/AMM.303-306.654

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February 2013

Authors:

Xiao Ling Zhang, Hui Juan Yu

Keywords:

Crocin Analogues, Gardenia jasminoides Ellis, Single-Walled Carbon Nanotubes, SPE

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References

[1] W. Tang, G. Eisenbrand, Chinese Drug of Plant Origin, Springer–Verlag, Berlin, (1992).

Google Scholar

[2] C. Dufresne, F. Cormier, S. Dorion, U.A. Niggli, S. Pfister, H. Pfander,. Enzyme. Microb. Technol. 24 (1999) 453.

DOI: 10.1016/s0141-0229(98)00143-4

Google Scholar

[3] F. Tubaro, E. Micossi, F. Ursini, J. Am. Oil. Chem. Soc. 73 (1996) 173.

Google Scholar

[4] T. Q. Pham, F. Cormier, E. Farnworth, V. H. Tong, M.R. Calsteren, J. Agric. Food Chem. 48 (2000) 1455.

Google Scholar

[5] S.W. Lee, J.M. Lim, S.H. Bhoo, Y.S. Paik, T.R. Hahn, Anal. Chim. Acta 480 (2003) 267.

Google Scholar

[6] J. Escribano, G.L. Alongso, M.C. Prados,. Cancer Lett. 100 (1996) 23.

Google Scholar

[7] F.I. Abdullaev, L.R. Negrete, H.C. Ortega, Toxicol. in Vitro 17 (2003) 731.

Google Scholar

[8] T. Ochiai, S. Ohno, S. Soeda, H. Tanaka, Y. Shoyama, H. Shimenl, Neurosci. Lett. 362 (2004) 61.

Google Scholar

[9] J.L. Rios, M.C. Recio, R.M. Giner, S. Manez, Phytother. Res. 10 (1996) 189.

Google Scholar

[10] K. Abe, M. Sugiura, S. Ymaguchi, Y. Shoyama, H. Saito, Brain Res 787 (1998) 132.

Google Scholar

[11] A. Kazuho, S. Hiroshi, Phytother. Res. 14 (2000) 149.

Google Scholar

[12] K. Premkumar, S.K. Abraham, S.T. Santhiya, A. Ramesh, Phytother. Res. 17 (2003) 614.

Google Scholar

[13] S. Soeda, T. Ochiai, L. Paopong, H. Tanaka, Y. Shoyama, H. Shimeno, Life Sci. 69 (2001) 2887.

DOI: 10.1016/s0024-3205(01)01357-1

Google Scholar

[14] Q. Yang, X.L. Zhang, J.X. Zhang, C.X. Fang, C.Y. Zheng. Manuscript in preparation.

Google Scholar

[15] X. Liu, Z.Y. Qia, J. Fu, J. China Pharmaceutical University. 27 (1996) 734.

Google Scholar

[16] Sigma 2011 catalogue, Product Number: S8381-1G http: /www. sigmaaldrich. com/catalog/search/ProductDetail/SIGMA/S8381.

Google Scholar