An Immunonanosilver Surface-Enhnaced Resonance Raman Scattering Method for Determination of Human Chorionic Gonadotrophin

Abstract:

In pH 6.6 Na2HPO4-citric acid buffer solution and in the presence of KCl, the nanosilver-labeled rabbit anti-hCG (Ag-RAhCG) was aggregated un-specifically to the aggregations. Upon addition of rhodamine 6G (RhG) molecular probe, it adsorb on the surface of Ag-RAhCG aggregations that exhibited the strongest surface-enhnaced resonance Raman scattering (SERRS) peak at 613 cm-1. In the optimal condition, the decreased SERRS intensity responds linearly with the concentration of hCG over 0.05-1.75 µg/mL. Based on this, a new and simple SERRS method has been proposed for the determination of hCG in serum samples, with satisfactory results.

• Periodical: Applied Mechanics and Materials (Volume 319)
• Pages: 157-160
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.319.157
• Online since: May 2013
• Authors: Xin Hui Zhang, Kun Li, Le Ning Hu, Ai Hui Liang, Zhi Liang Jiang
• Keywords: Human Chorionic Gonadotrophin, Immunoassay, Nanosilver-Labeled Anti-hCG, Surface-Enhaced Resonance Raman Scattering

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