PCR Detection of Mycobacterium bovis DNA in Cattle Bloods

Abstract:

For molecular biological detection of Mycobacterium tuberculosis, PCR methods with primers targeting different regions specific for mycobacterium tuberculosis complex are used worldwide. In this study,256 whole blood samples were detected using multiplex PCR.2 of all samples were found to be PCR positive. Homogenous comparison of Mycobacterium bovis specific genes with Mycobacterium bovis AF2122/97 all reached up to 99%.

• Periodical: Applied Mechanics and Materials (Volume 319)
• Pages: 169-173
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.319.169
• Online since: May 2013
• Authors: Chun Fang Wang, Yun Hang Gao, Xiu Yun Jiang, Jian Wang, Gong Mei Li, Fei Fei Chen, Ai Dong Qian
• Keywords: Cattle Bloods, Detection, Mycobacterium bovis, PCR

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