This research paper presents an experimental investigation on the tensile strength loss rate of glass fiber reinforced 2D woven fabric with ladder splicing laminate composites. On the basis of three kinds of ply sequence, two series of laminates with different plies numbers were prepared through RTM molding technology, including ladder splicing(LS) laminates and continuous laminates (CL). And the RTM technological parameters of RTM processing were designed as follows: injection temperature was room temperature, injection duration time was 180min and injection pressure was 0.5MPa, the epoxy resin based 2D glass fiber woven fabric RTM laminated specimen were prepared. According to the GB/T 1447-2005 standard test method, the tensile tests of the 2D glass fiber woven fabric laminates were carried out. Results show that the tensile strength loss rate of ladder splicing laminates is 11.05%, 9.37%, 7.78% while the number of plies is 8, 10, 12 respectively. In other words, the tensile strength loss rate is reduced with the plies number increasing. In addition, because of the concentration of tensile stress, all fractures of ladder splicing laminates occur at the site of splicing line.