Feasibility Experiment Investigation on Cell Microcapsule with Digital Micro-Fluidics Technology

Abstract:

We segmented the solution of sodium alginate into droplets and prepared alginate microcapsule with digital micro-fluidics technique. The Result microcapsule size , compared with the production obtained from pressure extrusion technology, is very uniformity and is distributed in a smaller range. The sample relative standard deviation is only 3.66.The size of alginate microcapsule can easily be controlled by changing the diameter of the nozzle. Furthermore, we can control the size of the alginate microcapsule simply by changing the drive voltage. The results show that this technique is more suitable for cell encapsulation in tissue engineering, cell therapy and biopharmaceutical applications, and provides a solution to the problem of controlling of uniformity, size and membrane thickness.

• Periodical: Applied Mechanics and Materials (Volumes 347-350)
• Pages: 1211-1215
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.347-350.1211
• Online since: August 2013
• Authors: Chuan Bo Huang
• Keywords: Cell, Digital Micro-Fluidics, Micro-Encapsulation, Sodium Alginate

References

[1] Chang TM. 2005. Therapeutic applications of polymeric artificial cells. Nat Rev Drug Discov 4: 221–235.

DOI: 10.1038/nrd1659

[2] Aldo Leal-Egaña, Anatol Fritsch. Tuning liver stiffness against tumours: An in vitro study using entrapped cells in tumour-like microcapsules [J]. Journal of the Mechanical Behavior of Biomedical Materials, 2012, 9(5): 113-121.

DOI: 10.1016/j.jmbbm.2012.01.013

[3] Salmons B, Gunzburg W H, Orive G, et al. Therapeutic application of cell microencapsulation in cancer[J]. Landes Bioscience, Austin, 2009: 92–103.

[4] Juan Dubrot, Aitziber Portero, Gorka Orive, et al. Delivery of immunostimulatory monoclonal antibodies by encapsulated hybridoma cells[J]. Cancer Immunology, Immunotherapy, 2010, 59(11): 1621-1631.

DOI: 10.1007/s00262-010-0888-z

[5] Jing Xiao, Ying Zhang, Jianzheng Wang, et al. Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay[J]. Applied Biochemistry and Biotechnology, 2010, 162, (7): 1996-(2007).

DOI: 10.1007/s12010-010-8975-3

[6] Guang-wei Sun, Xiao-xi Xu, Xiao-jun Ma et al. Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells[M]. Springer Protocols Handbooks, 2012, Human Embryonic and Induced Pluripotent Stem Cells, Part 1: 51-58.

DOI: 10.1007/978-1-61779-267-0_5

[7] Kim SK, Yu SH, Son JH, Hu¨bner H, Buchholz R. 1998. Calculations on O2 transfer in capsules with animal cells for the determination of maximum capsules size without O2 limitation. Biotechnol Lett 20: 549–552.

[8] Weiyi Zhang, Liya Hou. 2006. Scientific and Technological Problems of Digitalization of Micro-fluidics (Part II): Conceptual Study of Digitalization of Matter and Integrated Digitalization of Matter-Energy-Information. Science & Technology Review, 2006, 24(03): 41- 47.