In Situ Saccharification of Cellulose in Mild Ionic Liquid Using Sodium Alginate Immobilized Cellulase

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The work is to select biocompatible ionic liquid (IL) toward in situ saccharification of cellulose and investigating the effect of enzymatic saccharification with sodium alginate immobilized cellulase. The [Mmi [DM was selected for the ionic liquid treatment improved the yield of reducing sugars and the hydrolyzates could be efficiently fermented to ethanol. The yield of reducing sugar is 89.54% for 48h. In the in situ saccharification process, the yield of sugars were 84.52% and 86.72% with immobilized cellulase and free cellulase saccharification for 48h. Then the hydrolyzates could be fermented to ethanol with Candida shehatae. The yield of ethanol was 0.42g/g glucose within 24h.

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339-342

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August 2013

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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