Manufacturing Technique and Antimicrobial Activity of Silver Nanoparticles

Abstract:

Recently, the biomaterial is in rapid development stage, which could be widely applying in medical application, due to its biodegradability, nontoxic and biocompatibility. Chitosan is naturally abundant polymers have the biodegradability, nontoxic and biocompatibility. In this research, the chitosan and silver nitrate were used to develop the antibacterial agent via nanotechnique. In the reaction system, the reaction time and stirring speed were discussed, which will affect the surface plasmon resonance. The particle sizes were measured using transmission electron microscopy (TEM) and UV visible spectrophotometry. The silver nanoparticles size was below 100 nm via TEM. In addition, the results of antimicrobial activity indicated that the antibacterial agent has well antimicrobial activity on staphylococcus aureus. Due to the silver nanoparticle provides relatively higher surface area to contact with bacteria affect DNA molecules by losing their replication abilities.

• Periodical: Applied Mechanics and Materials (Volumes 365-366)
• Pages: 1169-1172
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.365-366.1169
• Online since: August 2013
• Authors: An Pang Chen, Ching Wen Lou, Ya Yuan Chung, Mei Chen Lin, Jia Horng Lin
• Keywords: Antimicrobial Activity, Chitosan, Nanoparticle, UV-Visible Spectrometer

References

[1] X. Ye, J.F. Kennedy, B. Li, B.J. Xie, Condensed state structure and biocompatibility of the konjac glucomannan/chitosan blend films, Carbohyd. Polym. 64 (2006), 532–538.

DOI: 10.1016/j.carbpol.2005.11.005

[2] M. Rinaudo, Chitin and chitosan: Properties and applications, Prog. Polym. Sci. 31 (2006), 603–632.

[3] T.C. Yang, C.C. Chou, C.F. Li, Antibacterial activity of N-alkylated disaccharide chitosan derivatives, Int. J. Food Microbiol. 97 (2005), 237–245.

DOI: 10.1016/s0168-1605(03)00083-7

[4] A. Hebeish, F.A. Abdel-Mohdy, Moustafa M.G. Fouda, Z. Elsaid, S. Essam, G.H. Tammam, Ehab A. Drees, Green synthesis of easy care and antimicrobial cotton fabrics, Carbohyd. Polym. 86 (2011), 1684–1691.

DOI: 10.1016/j.carbpol.2011.06.086

[5] R.E. Kesting, Semipermeable membranes of cellulose acetate for desalination in the process of reverse osmosis. II. Parameters affecting membrane gel structure, Applied Polymer, 9 (1965), 1873-1893.

DOI: 10.1002/app.1965.070090521

[6] A. Chhatre, P. Solasa, S. Sakle, R. Thaokar, A. Mehra, Color and surface plasmon effects in nanoparticle systems: Case of silver nanoparticles prepared by microemulsion route, Colloid. Surface A. 404 (2012), 83–92.

DOI: 10.1016/j.colsurfa.2012.04.016

[7] M. Sastry, K.S. Mayya, V. Patil, D.V. Paranjape, S.G. Hegde, J. Phys, Langmuir−Blodgett Films of Carboxylic Acid Derivatized Silver Colloidal Particles:  Role of Subphase pH on Degree of Cluster Incorporation, American Chemical Society, Chem. B101(1997).

DOI: 10.1021/jp964087f

[8] R.R. Naik, S.J. Stringer, G. Agarwal, S.E. Jones, M.O. Stone, Biomimetic synthesis and patterning of silver nanoparticles, Nature Publishing Group, 1 (2002), 169.

DOI: 10.1038/nmat758

[9] L.E. Murillo, O. Viera , E. Vicuna, J.G. Briano, M. Castro, Y. Ishikawa, R. Irizarry and L. Sola, Technical Proceedings of the 2002 International Conference on Computational Nanoscience and Nanotechnology, Chpt 16: Material and Nanostructures Science, Growth Kinetics of Gold Nanoparticles, Nanotech , 2 (2002).

[10] R.J. Murphy, D. Pristinski, K. Migler, J.F. Douglas, and V.M. Prabhu, Dynamic light scattering investigations of nanoparticle aggregation following a light-induced pH jump, J. Chem. Phys, 132 (2010), 194903.

DOI: 10.1063/1.3425883

[11] J. Hu, W. Lee, W. Cai, L. Tong and H. Zeng, Evolution of the Optical Spectra of Ag/mesoporous SiO2 Nanostructure Heat-treated in Air and H2 Atmospheres, Nanotechnology , 18 (2007), 185710.

DOI: 10.1088/0957-4484/18/18/185710

[12] Q.L. Feng, J. Wu, G.Q. Chen, F.Z. Cui, T.N. Kim, J.O. Kim, A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus, Journal of Biomedical Materials Research, 52 (2000), 662-668.

DOI: 10.1002/1097-4636(20001215)52:4<662::aid-jbm10>3.0.co;2-3

[13] J.S. Kim, E. Kuk, K.N. Yu, J.H. Kim, S.J. Park, H.J. Lee, S.H. Kim, Y.K. Park, Y.H. Park, C.Y. Hwang, Y.K. Kim, Y.S. Lee, D.H. Jeong and M.H. Cho, Antimicrobial effects of silver nanoparticles, Nanomedicine: Nanotechnology Biology and Medicine , 3 (2007).

DOI: 10.1016/j.nano.2006.12.001