The Effect and Mechanism of Apoptosis Induced by Desacetylcinobufotalin (DEBF) in Human Hepatocellular Carcinoma HepG2 Cells

Xu Chao; Lin Dang; Min Hui Wei

doi:10.4028/www.scientific.net/AMM.395-396.587

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 395-396The Effect and Mechanism of Apoptosis Induced by...

The Effect and Mechanism of Apoptosis Induced by Desacetylcinobufotalin (DEBF) in Human Hepatocellular Carcinoma HepG2 Cells

Abstract:

The cytotoxicity of Desacetylcinobufotalin (DEBF) and apoptosis induced by DEBF was measured. Additionally the mechanism of Apoptosis induced by DEBF was studied through Western blot. The results show DEBF displayed the marked inhibition effect to HepG2 cells and the IC₅₀ value is 0.0279μmol/ml. The expression of Bax was significantly increased and the expression of Bcl-2 was markedly decreased, compared to the control. The data suggest DEBF had significant antitumor activity through induction apoptosis via mitochondria pathway.

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Applied Mechanics and Materials (Volumes 395-396)

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587-590

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https://doi.org/10.4028/www.scientific.net/AMM.395-396.587

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September 2013

Authors:

Xu Chao, Lin Dang, Min Hui Wei

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Apoptosis, Desacetylcinobufotalin, HepG2

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