Study of Extraction of Total Alkaloids from Caulophyllum Robustum

Jian Lan Suo

doi:10.4028/www.scientific.net/AMM.395-396.601

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 395-396Study of Extraction of Total Alkaloids from...

Study of Extraction of Total Alkaloids from Caulophyllum Robustum

Abstract:

Objective: Optimizing the extraction of total alkaloids from Caulophyllum robustum. Methods: The taspine which is a type of alkaloids of Caulophyllum robustum is selected as the target index. First, developing the HPLC analysis protocol; second, using orthogonal design method to optimize the hydrochloric acid leaching protocol; third, using cation exchange column to isolate the alkaloids and examining the optimal conditions. Results: the optimal conditions of hydrochloric acid leaching protocol is using 7 column volume of 1% hydrochloric acid for 3 times. The materials are loaded onto the LSD001 cation exchange column with optimal pH 4 and flowrate 2ml/mL, the elution buffer is 10 column volume 5% hydrochloric acid. After desalting, the yield of alkaloids is 1.35% which includes 6.83% of taspine.

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Periodical:

Applied Mechanics and Materials (Volumes 395-396)

Pages:

601-604

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.395-396.601

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Online since:

September 2013

Authors:

Jian Lan Suo

Keywords:

Alkaloid, Cationexchange, Caulophyllumrobustum, High Performance Liquid Chromatography (HPLC)

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References

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