Influences of Organic Compounds on Laccase Activity Tests

Abstract:

Organic compounds oxalic acid and ethylenediaminetetraacetic acid disodium salt-2-hydrate (EDTA Na₂) were described as laccase inhibitors by forming complex compounds with the metal ions of the laccase. Their influence on laccase from Trametes hirsuta lg-9 and Rhus vernificera in different test systems utilizing 2, 2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2, 6-dimethoxyphenol (DMP) as enzyme substrates were tested. Our study indicated that oxalic acid can influence the laccase activity determination mainly by changing the pH of the reaction system. The influences of both oxalic acid and EDTA on the laccase activity determination with different substrates were different. The results indicated that both oxalic acid and EDTA could influence the laccase activity determination by influencing and the binding of laccases substrates but not by chelating metal of the laccase. The organic compounds can also influence the laccase activity determination by changing the pH of the reaction system.

• Periodical: Applied Mechanics and Materials (Volumes 416-417)
• Pages: 1702-1707
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.416-417.1702
• Online since: September 2013
• Authors: Ying Long Zhang, Hai Bo Zhang, You Shuang Zhu, Ming Le Cao, Ming Qiang Ai, Feng Huang
• Keywords: Inhibitor, Laccase, Rhus Vernificera, Substrate, Trametes hirsuta

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