Determination of Sodium Salvia Miltiorrhiza’s Optimal Analytical Conditions Detected by HPLC Method

Abstract:

The optimal analytical conditions of sodium salvia miltiorrhiza are detected by HPLC methods. SinoChrom ODS-BP C18 is selected as chromatographic column. The optimal experimental conditions under 280 nm wavelength UV detector include the column temperature: 28°C, ratio of methanol/water: 25/75 (v/v), and flow rate: 1.0ml/mol. The correlation coefficient is 0.9983, and RSD is 1.4%, when the linear range is 0.15~1.8μg/μL. In the experiment, the method is verified to be simple and reliable, and which can be used for the quality control in process of salvia miltiorrhiza extraction and purification.

• Periodical: Applied Mechanics and Materials (Volumes 446-447)
• Pages: 235-239
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.446-447.235
• Online since: November 2013
• Authors: Tao Wang
• Keywords: HPLC, Mobile Phase, Optimal, Sodium Salvia Miltiorrhiza

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