Effect of Solvent Composition on the Formation of Hydroxyapatite/Silk Fibroin Composites Prepared Using Sol-Gel Method

Abstract:

Hydroxyapatite (HA) has been widely utilized in the biomedical applications due to its chemical and structural features that are similar to the natural bones. The addition of organic components enhances the flexibility of the HA-based composites which result in increasing its molding ability into any desirable shapes. In this article, preparation of hydroxyapaptite/silk fibroin (HA/SF) composite using sol-gel method is reported. The optimal condition for preparing the HA/SF composites was determined by judging from their crystallite size, crystallinity and particle size distribution, morphology and calcium/phosphorus (Ca/P) ratio investigated with X-ray diffraction analysis, particle size analyzer, scanning electron microscopy and energy dispersive X-ray spectroscopy, respectively. The HA/SF composite was successfully prepared in the binary solvent of ethanol and water at the optimal volume ratio of 4:1. At this solvent condition, the composites had a uniform rod-liked shape, ranging from 30-70 nanometers. The Ca/P ratios of all composites are close to the theoretical value of about 1.67.

• Periodical: Applied Mechanics and Materials (Volumes 446-447)
• Pages: 408-413
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.446-447.408
• Online since: November 2013
• Authors: Suchaya Sriudom, Hataichanoke Niamsup, Surin Saipanya, Ruangsri Watanesk, Surasak Watanesk
• Keywords: Hydroxyapatite (HA), Silk Fibroin, Sol-Gel Method

References

[1] K. Ozeki, H. Aoki, T. Masuzawa, Influence of the hydrothermal temperature and pH on the crystallinity of a sputtered hydroxyapatite film, Appl. Surf. Sci. 256 (2010) 7027-7031.

DOI: 10.1016/j.apsusc.2010.05.018

[2] I.M. Pelin, S.S. Maier, G.C. Chitanu, V. Bulacovschi, Preparation and characterization of a hydroxyapatite-collagen composite as component for injectable bone substitute, Mater. Sci. Eng. C 29 (2009) 2188-2194.

DOI: 10.1016/j.msec.2009.04.021

[3] S.B. Kim, Y.J. Kim, T.L. Yoon, S.A. Park, I.H. Cho, E.J. Kim, I.A. Kim, J-W. Shin, The characteristics of a hydroxyapatite-chitosan-PMMA bone cement, Biomaterials 25 (2004) 5715-5723.

DOI: 10.1016/j.biomaterials.2004.01.022

[4] M.C. Chang, C-C. Ko, W.H. Douglas, Preparation of hydroxyapatite-gelatin nanocomposite, Biomaterials 24 (2003) 2853-2862.

DOI: 10.1016/s0142-9612(03)00115-7

[5] M.H. Fathi, A. Hanifi, V. Mortazavi, Preparation and bioactivity evaluation of bone-like hydroxyapatite nanopowder, J. Mater. Process. Technol. 202 (2008) 536-542.

DOI: 10.1016/j.jmatprotec.2007.10.004

[6] J.S. Cho, S-H. Rhee, Formation mechanism of nano-sized hydroxyapatite powders through spray pyrolysis of a calcium phosphate solution containing polyethylene glycol, J. Europ. Ceram. Soc. 33 (2013) 233-241.

DOI: 10.1016/j.jeurceramsoc.2012.08.029

[7] X. Li, X. Wang, A. Ito, Y. Sogo, K. Cheng, A. Oyane, Effect of coprecipitation temperature on the properties and activity of fibroblast growth factor-2 apatite composite layer, Mater. Sci. Eng. C 29 (2009) 216-221.

DOI: 10.1016/j.msec.2008.06.012

[8] J. Liu, X. Ye, H. Wang, M. Zhu, B. Wang, H. Yan, The influence of pH and temperature on the morphology of hydroxyapatite synthesized by hydrothermal method, Ceram. Int. 38 (2003) 629-633.

DOI: 10.1016/s0272-8842(02)00210-9

[9] A. Bigi, E. Boanini, K. Rubini, Hydroxyapatite gels and nanocrystals prepared through a sol-gel process, J. Solid State Chem. 177 (2004) 3092-3098.

DOI: 10.1016/j.jssc.2004.05.018

[10] H. Bundela, A. K Bajpai, Desinging of hydroxyapatite gels-gelatin based porous matrix as bone substitute: Correlation with biocompatibility aspects, Express. Polym. Lett. 2 (2008) 201-213.

DOI: 10.3144/expresspolymlett.2008.25

[11] T. Goto, I.Y. Kim, K. Kikuta, C. Ohtsuki, Hydroxyapatite formation by solvothermal treatment of α-tricalcium phosphate with water-ethanol solution, Ceram. Int. 38 (2012) 1003-1010.

DOI: 10.1016/j.ceramint.2011.08.023

[12] X. Guo, P. Xiao, Effects of solvent on properties of nanocrystalline hydroxyapatite produced from hydrothermal process, J. Eur. Ceram. Soc. 26 (2006) 3383-3391.

DOI: 10.1016/j.jeurceramsoc.2005.09.111

[13] F. Nagata, Y. Yokogawa, M. Toriyama, Y. Kawamoto, T. Suzuki, K. Nishizawa, Hydrothermal synthesis of hydroxyapatite crystals in the presence of methanol, J. Ceram. Soc. Jpn. 109 (1995) 70-73.

DOI: 10.2109/jcersj.103.70

[14] Y. Yang, F. Ding, J. Wu, W. Hu, W. Liu, J. Liu, X Gu, Development and evaluation of silk-fibroin-based nerve grafts used for peripheral nerve regeneration, Biomaterials 28 (2007) 5526-5535.

DOI: 10.1016/j.biomaterials.2007.09.001

[15] T. Tsuchida, J. Kubo, T. Yoshioka, S. Sakuma, T. Takeguchi, W. Ueda, Reaction of ethanol over hydroxyapatite affected by Ca/P ratio of catalyst, J. Catal. 259 (2008) 183-189.

DOI: 10.1016/j.jcat.2008.08.005