Rapid Dissociation of Cortical Neurons from Neonate Rats and Patch-Clamp Recording

Abstract:

In order to get the single cortical neurons fitting the study of patch-clamp technique.use the kunming mouse with the birth of 10 days or so, and separate neurons in the cerebral cortex through acute separation technology and enzymatic hydrolysis. A cone-shaped adherent neurons are selected and observed in rapid dissociation and cell activity status under the inverted microscope. Different ion channel blockers is added to the extracellular fluid and used whole-cell recording which can obtain Na ⁺, K ⁺ and other ion channel currents. This method can obtain good morphological and physiological characteristics of both single cortical neurons, confirming that the method applied to cortical neurons by patch clamp technique, which has significant value for in-depth research on drugs, toxins and other physical factors on the electrical activity of neuronal ion channels and signal transduction mechanism of the effect.

• Periodical: Applied Mechanics and Materials (Volumes 446-447)
• Pages: 427-433
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.446-447.427
• Online since: November 2013
• Authors: Xiao Lin He, Jin Hai Wang, Yu Zheng, Yang Zhang, Gang Li
• Keywords: Ion Channels, Neurons, Patch Clamp Technique, Rapid Dissociation, Whole-Cell Recording

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