Study on the Degradation of Chlorpyrifos by Immobilized Bacillus

Abstract:

Pesticide pollutions have become increasingly severe. Especially the extensive production and application of chlorpyrifos have resulted in the environmental pollution and ecological damage. The employment of the immobilized microorganism degradation of chlorpyrifos in soil is a kind of effective technology without secondary pollution. The best ratio of immobilized carrier has been determined by comparison among different carriers for chlorpyrifos degradation. Based upon the adoption of Bacillus LLBD4, pH value, temperature and initial concentration of chlorpyrifos were studied in this experiment. The results showed that the combination of 10% of PVC, 1% of activated carbon and 0.5% sodium alginate can be served as the best carrier, furthermore, chlorpyrifos degradation efficiency reached the peak when the pH was set to 7.0, the initial concentration of chlorpyrifos was 100 mg·L^-1 at 30°C, reaching about 83%.

• Periodical: Applied Mechanics and Materials (Volumes 448-453)
• Pages: 613-619
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.448-453.613
• Online since: October 2013
• Authors: Xin Wang*, Jia Liang Lu, Li Liu, Jia Bao, Jiyan Liang, Li Cui
• Keywords: Chlorpyrifos, Degradation, Immobilized

References

[1] Pandey S, Singh D K. Soil dehydrogenase, phosphomonoesterase and arginine demitasse activities in an insecticide treated groundnut (Arachis hypogaeaL. ) field [J]. Chemosphere, Forum Vol. 63(2006), pp.869-880.

DOI: 10.1016/j.chemosphere.2005.07.053

[2] Chu X Q , Fang H, Pan X D, et al . Degradation of chlorpyrifos alone and in combination with chlorothalonil and their effects on soil microbial populations [J]. Journal of Environmental Sciences, Forum Vol. 20(2008), pp.464-469.

DOI: 10.1016/s1001-0742(08)62080-x

[3] Dutta M , Sardar D , Pal R, et al. Effect of chlorpyrifos on microbial biomass and activities in tropical clay loam soil [J]. Environmental Monitoring and Assessment, Forum Vol. 160(2010), pp.385-391.

DOI: 10.1007/s10661-008-0702-y

[4] De Silva P M, Pathiratne A , van Straalen N M . et al. Chlorpyrifos causes decreased organic matter decomposition by suppressing earthworm and termite communities in tropical soil[J]. Environmental Pollution, Forum Vol. 158(2010), pp.3041-3047.

DOI: 10.1016/j.envpol.2010.06.032

[5] Yuze Wang, Qixing Zhou. Soil micro-interfacial processes of organic pollutants and related affecting factors. Chinese Journal of ecology, Forum Vol. 31(2012), pp.1034-1042, (in Chinese).

[6] D'Souza SF. Trends in immobilized enzyme and cell technology. Indian J Biotechnol, Forum Vol. 1(2002), pp.321-328.

[7] Cassidy MB, Lee H, Trevors JT. Environmental applications of immobilized microbial cells: a review. J Ind Microbiol, Forum Vol. 16(1996), pp.79-101.

[8] Gita Kulshrestha, Anupriya Kumari. Fungal degradation of chlorpyrifos by Acremonium sp. strain(GFRC-1) isolated from a laboratory-enriched red agricultural soil . Biol Fertil Soils, Forum Vol. 47(2011), pp.219-225.

DOI: 10.1007/s00374-010-0505-5

[9] P. N. Tallur , V. B. Megadi , H. Z. Ninnekar. Biodegradation of p-cresol by immobilized cells of Bacillus sp. strain PHN 1. Biodegradation, Forum Vol. 20(2009), pp.79-83.

DOI: 10.1007/s10532-008-9201-7

[10] Rahman R. N., Ghazali F. M. and Salleh A. B, Biodegradation of hydrocarbon contamination by immobilized bacterial cells . The Journal of Microbiology, Forum Vol. 44(2006), p.354–359.

[11] Nurdan K P, Merih S A T. Treating denim fabrics with immobilized commercial cellulases [J]. Process Biochemistry, Forum Vol. 40(2005), pp.767-771.

DOI: 10.1016/j.procbio.2004.02.003

[12] Xin Wang, Peijun Li, Zongqiang Gong. Remediation of soil contaminated with benzo (α) pyrene using mixed immobilized yeast strains . Environment control and pollution, Forum Vol. 30(2008), pp.1-8.

[13] Yunhan Mao, Jianlong Wang, Immobilization of activated sludge in PVA matrix using innovative methods [J]. Acta Scientiae Circumstantiae, Forum Vol. 33 (2013), pp.370-376.

[14] Hai-ming Duan. Biodegradation characteristics of chlorpyrifos by sodium alginate immobilized bacteria . Chinese Journal of Eco-Agriculture, Forum Vol. 20(2012), pp.1636-1642.

DOI: 10.3724/sp.j.1011.2012.01636