Study on the Conductivity of DMEM by PASCO Sensors

Article Preview

Abstract:

The fusion solution is a significant factor for the effect of the cell electrofusion, traditional buffer solution is different from the cell culture solution, researchers would spend lots of time and money to move the cells from the culture solution (DMEM) into the fusion buffer. The aim of this study is to explore a new formula on the basis of DMEM solution instead of Zimmerman solution. Theoretically its feasible to instead of fusion solution when the ratio of DMEM solution and water is less than 9:500.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

158-160

Citation:

Online since:

February 2014

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2014 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

[1] Ning Hu, Jun Yang, Sang W. Joo, Arghya Narayan Banerjee, Shizhi Qian. Sensors and Actuators B: Chemical, 2013(178): 63-85.

Google Scholar

[2] Zhen Yu Wang, Jun Yang, Ning Hu, Xiao Lin Zheng, Zhong Yang. Chinese Journal of Analytical Chemistry, 2012(40): 331-338.

Google Scholar

[3] Marko Usaj, Karel Flisar, Damijan Miklavcic, Masa Kanduser. Bioelectrochemistry, 2013(92): 47.

DOI: 10.1016/j.bioelechem.2013.01.003

Google Scholar

[4] Chen E H, Grote E, MohlerW, Vignery A. FEBS Lett. , 2007, 581: 2181~ 2193.

Google Scholar

[5] A. Stromberg, F. Ryttsen, D.T. Chiu, M. Davidson, P.S. Eriksson, C.F. Wilson, O. Orwar, R.N. Zare, Manipulating the genetic identity and biochemical surface properties of individual cells with electric-field-induced fusion, Proceedings of the National Academy of Sciences of the United States of America 97 (2000).

DOI: 10.1073/pnas.97.1.7

Google Scholar

[6] U. Zimmermann, J. Vienken, G. Pilwat, W.M. Arnold, Electro-fusion of cells: principles and potential for the future, Ciba Found Symp. 103 (1984) 60–85.

DOI: 10.1002/9780470720844.ch5

Google Scholar

[7] U. Zimmermann, J. Vienken, Electric field-induced cell-to-cell fusion, Journal of Membrane Biology 67 (1982) 165–182.

DOI: 10.1007/bf01868659

Google Scholar

[8] Robl, J. M. J. Anim. Sci, 64: 642-647, (1987).

Google Scholar

[9] Kubiak, J. Z. et al: Exp. Cell Res, 157: 561-566, (1985).

Google Scholar

[10] B. Oback, D.N. Wells, Cloning cattle, Clon. Stem Cells 5 (2003) 243–256.

DOI: 10.1089/153623003772032763

Google Scholar

[11] B. Oback, A.T. Wiersema, P. Gaynor, G. Laible, F.C. Tucker, J.E. Oliver, A.L. Miller, H.E. Troskie, K.L. Wilson, J.T. Forsyth, M.C. Berg, K. Cockrem, V. McMillan, H.R. Tervit, D.N. Wells, Cloned cattle derived from a novel zona-free embryo reconstruction system, Clon. Stem Cells 5 (2003).

DOI: 10.1089/153623003321512111

Google Scholar

[12] Lee SW, Choi JH, Kim YK. Analysis and experimental investigation of dielectrophoretic force in a planar electrode structure. J Micromech Microeng. 1996: 66-68.

DOI: 10.1088/0960-1317/6/1/014

Google Scholar