This study is to measure the impact pressure of high-speed water jet injected in water at the stand-off distance from the nozzle exit. The high-speed water jets are generated by the impact of a projectile, which known as impact acceleration method, launched by Horizontal Single Stage Power Gun. The maximum averaged jet velocity of about 374.24 m/s in water was generated in this experiment. The impact pressure of high-speed water jet in water at the stand-off distance 15, 20, 30 and 40 mm from the nozzle exit was measured by the PVDF pressure sensor. Moreover, the impact phenomena of the jet were visualized by a high-speed video camera with shadowgraph optical arrangement. From the pressure sensor, two peak over-pressures are always observed in this experiment. From visualization, it was found that the two peak over-pressures of 24 GPa and 35 GPa at x = 15 mm were generated by the jet and the bubble impact, respectively. The peak over-pressure decreases exponentially as the stand-off distance between the PVDF pressure sensor to the nozzle exit increases. Moreover, the jet and the bubble impact on the PVDF pressure sensor, shock waves, and bubble deformation were obviously observed in this study.