Biopsy precious research has an important relationship with treatment efficiency and side effect complication. Most of the investigation is focus on the non-creatural material which is used to substitute creatural soft tissue for precious research. The aim of this paper is to develop a prescription that can be used for opaque and transparent PVA making which has similar mechanics property to the creatural tissue. The preparation, characteristics, and the results of PVA hydrogel on the composite material are studied as the key investigation. Porcine liver tissue property is also tested as a consultant for PVA material preparation. Comparing with the liver tissue test result, certain component ratio and freezing-thaw circle are determined. This PVA hydrogel material shows big potential in the creatural material substitution application. This research will benefit others who are interested in using non-creatural material to do experiment or surgical technology training.