Molecular Cloning and Computational Analysis of Rice OsEm Gene

Abstract:

Late embryogenesis abundant (LEA) proteins play important roles in enhancing the resistance of plants to adverse stresses and promoting seeds development and maturation. The OsEm gene in rice is one of LEA protein-encoding genes, however, physical and chemical properties, phylogenetic relationship and protein-protein interaction network of it are still unknown. Here, the full length cDNA of OsEm was cloned using nested RT-PCR, the sequencing result was then computational analyzed using bioinformatics approaches. The results showed that OsEm encoded a high hydrophilic protein with small molecule weight, it is mainly composed of alpha helix, extended strand, beta turn, random coil and contains a conserved region with 20 amino acid residues. Phylogenetic analysis showed that OsEM protein has the nearest phylogenetic relationship to BiEM1, but has the distant phylogenetic relationship to BnEM6. Results of protein-protein interaction network showed the expression of OsEm gene was regulated by complexes of VP1/BZ8 or VP1/TRAB1. This work is helpful to further define the function of OsEm gene and provide useful information for improving the adverse stress tolerance of plants in the future.

• Periodical: Applied Mechanics and Materials (Volumes 522-524)
• Pages: 1109-1116
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.522-524.1109
• Online since: February 2014
• Authors: Jing Fan, Ming Yuan Huang, Xi Yu Zhang, Zi Liang, Cheng Hao Xu
• Keywords: Late Embryogenesis Abundant (LEA) Proteins, OsEm, Phylogenetic Analysis, Protein-Protein Interaction Network, Rice

References

[1] D. Xu, X. Duan, Wang B, B. Hong, T.H.D. Ho and R. Wu: Plant physiology. Vol. 110 (1996), p.249.

[2] L. DureIII, S.C. Greenway, and G.A. Galau: Biochemistry. Vol. 20 (1981), p.4162.

[3] E. Jaspard, D. Macherel and G. Hunault: PloS one. Vol. 7 (2012), p.1.

[4] M. Raynal, P. Gaubier and F. Grellet: Nucleic acids research. Vol. 18 (1990), p.6132.

[5] J.L. Ried and M.K. Walker-Simmons: Plant physiology. Vol. 102 (1993), p.125.

[6] M. Hundertmark and D.K. Hincha: BMC genomics. Vol. 9 (2008), p.1.

[7] P.S. Reddy, G.M. Reddy, P. Pandey, K. Chandrasekhar and M. K. Reddy: Molecular biology reports. Vol 39 (2012), p.7163.

[8] C. Krüger, O. Berkowitz, U.W. Stephan and R. Hell: Journal of Biological Chemistry. Vol. 277 (2002), p.25062.

[9] T.Z. Hu: Russian journal of plant physiology. Vol. 55 (2008), p.530.

[10] R. Rodríguez Valentín, F. Campos, M. Battaglia, R.M. Solórzano, M.A. Rosales and A.A. Covarrubias: Plant molecular biology reporter. Vol. 31(2013), p.1.

[11] T. Hu, H. Zeng, S. He, Y. Wu, G. Wang and X. Huang: Applied biochemistry and biotechnology. Vol. 166 (2012), p.222.

[12] J.C. Litts, M.B. Erdman, N. Huang, E.E. Karrer, A. Noueiry, R.S. Quatrano and R.L. Rodriguez: Plant molecular biology. Vol. 19 (1992), p.335.

DOI: 10.1007/bf00027357

[13] T. Hattori, T. Terada and S. Hamasuna: The Plant Journal, Vol. 7 (1995), p.913.

[14] K. Miyoshi, Y. Kagaya, Y. Ogawa, Y. Nagato and T. Hattori: Plant and cell physiology. Vol. 43 (2002), p.307.

[15] G.E. Crooks, G. Hon, J.M. Chandonia and S.E. Brenner: Genome Res. Vol. 14 (2004), p.1188.

[16] Y.G. Wang, J.Z. Ma, X.Q. Ma, J.G. Liu and M.J. Yang: Advanced Materials Research. Vol. 643 (2013), p.56.

[17] Y.M. Zhao, J.X. Yang and J.N. Yu: Journal of Xi' an University of Arts and Science (Nat Sci Ed). Vol. 9 (2006), p.27 (in chinese).

[18] X.S. Wang, H.B. Zhu, G.L. Jin, H.L. Liu, W.R. Wu and J. Zhu: Plant Science. Vol. 172 (2007), p.414.

[19] M. Ganguly, K. Datta, A. Roychoudhury, D. Gayen, D.N. Sengupta and S.K. Datta: Plant signaling & behavior. Vol. 7 (2012), p.502.

DOI: 10.4161/psb.19646

[20] C.M. Vicient, T.J. Roscoe and M. Delseny: Journal of experimental botany. Vol. 49 (1998), p.1061.

[21] X.Y. Zhu and B.Y. Shen: Letters in biotechnology. Vol. 15 (2004), p.70 (in chinese).

[22] K. Mukherjee, A.R. Choudhury, B. Gupta, et al: BMC plant biology. Vol. 6 (2006), p.1.

[23] H. Nakagawa, K. Ohmiya and T. Hattori: The Plant Journal. Vol. 9 (1996), p.217.

[24] S. Ramanjulu and D. Bartels: Plant, cell & environment. Vol. 25 (2002), p.141.

[25] T. Hobo, Y. Kowyama and T. Hattori: Proceedings of the National Academy of Sciences. Vol. 96 (1999), p.15348.