A Microfluidic Cytometer for Quantitative Evaluation of Radiation Dose by γ-H2AX

Abstract:

Evaluation of radiation dose is very important for the detection of radiation damage. γ-H2AX is a popular biological dosimeter to evaluate the radiation effect. Typically, bulky and expensive commercial flow cytometers are used to detect γ-H2AX. This paper presents a miniaturized and highly sensitive cytometer using a microfluidic chip for evaluating the radiation dose by detecting the mean immunofluorescence intensity of γ-H2AX. A compact optical focusing system and a shift-phase differential amplifier are designed to improve the detection sensitivity. Sample lymphocyte cells are stained by FITC fluorescent dye after being irradiated by UVC. Comparison experiments between the developed miniature cytometer and a commercial flow cytometer were conducted under different radiation doses. The developed microfluidic cytometer can also demonstrate a good linear correlation between the measured fluorescence intensity and the irradiation dose with a detection limit similar to that of the commercial flow cytometer. The developed cytometer can evaluate quantitatively the radiation dose by the mean fluorescence intensity of γ-H2AX with a significantly smaller amount of blood samples than a commercial flow cytometer.

• Periodical: Applied Mechanics and Materials (Volumes 522-524)
• Pages: 1119-1122
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.522-524.1119
• Online since: February 2014
• Authors: Jun Sheng Wang, You Nan Song, Jin Yang Sun, Hui Chu, Jin Hu Jiang, Xin Xiang Pan, Ye Qing Sun*, Dong Qing Li
• Keywords: Fluorescence Cytometer, Microfluidic Chip, Radiation Detection, γ-H2AX

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