Analysis of DNA Damages of Gonadal Cells of Hemicentrotus pulcherrimus in Petroleum Hydrocarbons

Abstract:

The single cell gel electrophoresis (SCGE) is a rapid and sensitive procedure for measuring strand breaks in DNA. In the present study, sea urchin (Hemicentrotus pulcherrimus) was chosen as the test organism and SCGE was applied to assess DNA damage of its gonadal cells exposed to petroleum hydrocarbon. The gonadal cells of sea urchin had been seriously damaged above 50 mg/L of Water Accommodated Fractions (WAFs), whileas no damages occurred in the lower concentrations. There were good linear relationships between exposure days and DNA damage rate, percentage of DNA in the comet tail (%TDNA) as well as comet tail length (TL).

• Periodical: Applied Mechanics and Materials (Volumes 522-524)
• Pages: 251-256
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.522-524.251
• Online since: February 2014
• Authors: Jun Song Han, Xin Lu Lv, Ya Li Gao, Xiang Gao, De Qi Xiong
• Keywords: Comet Tail Length, DNA Damage, Hemicentrotus ulcherrimus, Petroleum Hydrocarbons

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