Implementation of the Dynamic Tension Load of Cell Membrane and its Stress Analysis

Abstract:

Cell stress experimental study is currently a domestic and overseas research hotspot. Related research findings are widely applied to study the proliferation, activation, and functional status of cells when they are faced with deformation under physiological or pathological condition. It is of great significance to study the motion and status of cell and extra-cellular matrix under different stresses, for this may help us to further discover the re-build law of cell tissue, and to figure out corresponding remedy mechanism [. In order to disclose the relation between cell tissue proliferation activation result and the magnitude, property, affection duration and frequency of cell stress, so as to further reveal the proliferation and activation law of cell tissue, a dynamic stress with adjustable frequency and pressure is exerted. Hence, we developed a membrane-type variable stress wave cell membrane dynamic tensile stress test system [2-. The test system is designed to experimentally research several cell samples bearing stress from the same source at the same time, which is able to offer a required static pressure on tested cell tissue, as well as cell membrane experiment stress wave with certain variable frequency (0-20 times per minute) and adjustable magnitude. How to load dynamic tensile stress on cells is a key problem to be solved in cell culture test system. In allusion to the problem, a mechanical model is constructed in this paper, so as to analyze related mechanical features. On this basis, the stress and strain distribution law of various points on cell membrane is figured out. This has provided theoretical basis for area selection and design of cell seeding, experimental research on cell stress and result analysis.

• Periodical: Applied Mechanics and Materials (Volumes 541-542)
• Pages: 1497-1503
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.541-542.1497
• Online since: March 2014
• Authors: Wen Ji Hu*, Yu Yu, Ji Xiang Zhou, Xue Bin Tang
• Keywords: Distribution Law, Dynamic Tension Load, Elastic Membrane Displacement, Elastic Membrane Stress

References

[1] Zhou Jixiang, Zhao Zhihe, Tan Lijun, Lin Zhu. Effect of Different Tension Stress on the Proliferation Activity of Human Peridontal Ligament Fibroblast in Vitro. Journal of Sichuan University (Medical Edition), 2003, 34 (4), 638~640.

[2] Yu Yu, Qin Shuren, Deng Zhiping, Zhou Jixiang. Mechanics Characteristic Analysis and Improvement of Film Dynamic Stress Modulation. Journal of Chongqing University (Natural Science Edition), 2007. 8.

[3] Yu Yu, Qin Shuren, Deng Zhiping, Zhu Weibing, Zhou Jixiang. Creation of an Experimental System for Testing the Dynamic Tensile Stress of Cells Using Membrane Variable Stress Wave. Journal of Chongqing University. 2005, 28 (9): 1~5.

[4] Yu Yu, Jiang Yonghua, Sun Haishan, Zhou Jixiang. Design of an Experimental System for Testing the Dynamic Tensile Stress of Cells based on Microcontroller. Machinery Design & Manufacture. 2007. 5.

[5] Yang Guitong. Elastic Mechanics. Higher Education Press, (2003).

[6] Yang Guitong. Introduction to Elasticity and Plasticity. Tsinghua University Press. (2004).

[7] Hong Shuizong. Modern Testing Technology. Shanghai Jiao Tong University Press. (2002).