Spectrofluorimetric Determination of Bilirubin Using Enoxacine-Terbium Probe

Abstract:

A new spectrofluorimetric method was developed for determination of trace amount of bilirubin. Using enoxacine–terbium ion as a fluorescent probe, in the buffer solution of pH=5.8, BR can remarkably reduce the fluorescence intensity of the ENX-Tb³⁺ complex at λ=545nm and the reduced fluorescence intensity of Tb³⁺ ion is in proportion to the concentration of BR. Optimum conditions for the determination of BR were also investigated. The linear range and detection limit for the determination of BR are 1.0×10^-7～4.5×10^-6mol/L and 8.1×10^-8mol/L. This method is simple, practical and can be successfully applied to assess BR in serum samples.

• Periodical: Applied Mechanics and Materials (Volumes 556-562)
• Pages: 421-424
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.556-562.421
• Online since: May 2014
• Authors: Wei Wei Bian*
• Keywords: Bilirubin, Enoxacine, Spectrofluorimetry, Terbium

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