Biological Conjugate between Antibody and ZnS Nanoparticles as Probe for Atomic Absorption Spectrophotometry Determination of Estriol

Abstract:

ZnS semiconductor nanocrystals (NCs) were prepared by ways from primary materials of ZnCl₂ and Na₂S in water solution. Using the synthesized ZnS NCs, a polyclonal antibody-based ZnS-labelled immunosorbent assay for the determination of estriol (E₃) was developed with atomic absorption spectrophotometry (AAS) as a detector. An immunoaffinity column was applied to testify conjugation between antibody and ZnS NCs. The linear range for determination of estriol is 40.0~600.0 ng.mL^-1, and the limit of detection (LOD) is 10.0 ng.mL^-1. Some serum samples have been analyzed with satisfactory results which are in good agreement with those obtained using ELISA. This work suggests the potential application of NCs as biological probes and AAS as detector in nonisotopic immunoassay.

• Periodical: Applied Mechanics and Materials (Volumes 556-562)
• Pages: 64-66
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.556-562.64
• Online since: May 2014
• Authors: Chun Yan Zhang, Chuan Tao Wang, Shu Hao Wang, Ling Yun Du*
• Keywords: Atomic Absorption Spectrophotometry, Estriol, Immunoassay, Semiconductor Nanocrystals, ZnS

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