A Steroidal Saponin RCE-4 Inhibits Lipopolysaccharide-Stimulated Inflammatory Responses via Blocking PI3K/Akt-Mediated Nf-κB Activation in RAW264.7 Cells

Cai Hong Bai; Hai Bo He; Fan Cheng; Kun Zou; Jun Zhi Wang; Guang Yao Liu; Wei Deng; Zhi Feng Chen; Xiao Chen; Jian Feng Chen

doi:10.4028/www.scientific.net/AMM.568-570.1901

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 568-570A Steroidal Saponin RCE-4 Inhibits...

A Steroidal Saponin RCE-4 Inhibits Lipopolysaccharide-Stimulated Inflammatory Responses via Blocking PI3K/Akt-Mediated Nf-κB Activation in RAW264.7 Cells

Abstract:

Steroidal saponin: (1β,3β,5β,25S)-spirostan-1,3-diol1-[α-L-rhamnopyranosyl-(1→2)-β-D-xylopyranoside] (RCE-4) is the most abundant and bioactive members in Reineckia carnea, has been reported to possess antiinflammatory activity, but the underlying mechanisms remain largely unknown. The present aim was to study expression of inflammatory cytokines, on the basis of this investigation, the possible mechanism of RCE-4 was elucidated. In the present study, we found that the concentrations of TNF-α, IL-1β and IL-6 released from LPS-stimulated RAW264.7cells significantly increased compared to control (P<0.01, respectively). After pretreatment with RCE-4, the TNF-α, IL-1β and IL-6 levels significantly decreased compared with the LPS group (P<0.05, P<0.01, respectively). Further studies indicated that RCE-4 significantly suppressed Akt phosphorylation and NF-қB activation, and with the dose of RCE-4 increasing; their improvement became more and more strong. Our results showed that RCE-4 inhibited LPS-stimiulated TNF-α, IL-1β, IL-6 productions through the blockage of PI3K/Akt-mediated NF-κB activation. Our findings might provide a molecular basis for the ability of RCE-4 serving as a promising candidate for treating various inflammatory diseases.

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Periodical:

Applied Mechanics and Materials (Volumes 568-570)

Pages:

1901-1906

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.568-570.1901

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Online since:

June 2014

Authors:

Cai Hong Bai, Hai Bo He*, Fan Cheng, Kun Zou, Jun Zhi Wang, Guang Yao Liu, Wei Deng, Zhi Feng Chen, Xiao Chen, Jian Feng Chen

Keywords:

Nf-κB, PI3K/Akt, RAW264.7 Cells, Steroidal Saponin from Reineckia carnea

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