Visual Detection of Mycobacterium tuberculosis Complex with Loop-Mediated Isothermal Amplification and Eriochrome Black T

Abstract:

The present study was aimed to improve the method for detecting Mycobacterium tuberculosis complex recently reported on Letters in Applied Microbiology. Eriochrome black T (EBT) was added to the reaction system of LAMP, due to complexation of EBT with magnesium ions, the color of reaction solution was red at beginning, when the reaction was in process, the precipitate of magnesium ions and pyrophosphate ions formed, EBT lost magnesium ions, the color of reaction solution became sky blue from red gradually, and the method exhibits a high sensitivity of 8 fg/μL. The improved LAMP assay can detect Mycobacterium tuberculosis complex rapidly, sensitively and visually, the method developed in this study had solved the “bottleneck” in popularization and application of LAMP technology, which had not only avoided aerosol pollution but also solved the false positive problem caused by primer dimers.

• Periodical: Applied Mechanics and Materials (Volume 618)
• Pages: 264-267
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.618.264
• Online since: August 2014
• Authors: De Guo Wang*
• Keywords: Eriochrome Black T (EBT), Loop-Mediated Isothermal Amplification (LAMP), Mycobacterium tuberculosis Complex

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