Bismuth-Modified Hydroxyapatite Carbon Electrode for Heavy Metal Detection in Biomatrices

Abstract:

Micro-analytical based sensors with complex biomatrices such as blood, urine, or saliva are of increasing interest in health-care sector because of their sensitivity, selectively and simplicity. A modified carbon electrode with hydroxyapatite (HAp) and Bismuth (II) was developed to detect Pb²⁺ concentration in blood sera from healthy persons. Square wave anodic stripping voltammetry was used for the detection of Pb²⁺ by the interacting capability of the Bi film and HAp on the electrode surface with the optimised parameters of deposition time 240sec, deposition potential –1.0 V in 0.1M acetate buffer followed by square wave potential scan from-1.0 V to-0.2V. Bi film deposited HAp-CME showed attractive electrochemical characteristics with high sensitivity for Pb²⁺. Clear current peaks for different Pb²⁺ concentrations were observed around-0.55V, with favourable signal-to-background ratio, and comparatively free from oxygen interferences.

• Periodical: Applied Mechanics and Materials (Volume 625)
• Pages: 813-817
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.625.813
• Online since: September 2014
• Authors: Huma Ajab, Aamir Amanat Ali Khan, John Ojur Dennis, Mohd Azmuddin Abdullah
• Keywords: Bismuth, Blood Serum, Carbon Electrode, Electrochemical Sensor, Hydroxyapatite (HA)

References

[1] P. Jothimuthu, A. R. Wilson, J. H. Erin, N. H. William, R. H. Ian, Papautsky. Lab-on-a-chip sensor for detection of highly electronegative heavy metals by anodic stripping voltammetry. Biomed Microdevices. 13(4) (2011) 695-703.

DOI: 10.1007/s10544-011-9539-1

[2] Exposure to lead: A major public health concern. Geneva, World Health Organization, 2010. (http: /www. who. int/ipcs/features/lead. pdf, accessed 20 December 2010).

[3] P. Kissinger, W.R. Heineman, Laboratory Techniques in Electroanalytical Chemistry. New York and Basel (1996) 331-334.

[4] A. Krolicka, K. Bobrowski, J. Kalcher, I. Mocak, K. Svancara, Vytras, Study on Catalytic Adsorptive Stripping Voltammetry of Trace Cobalt at Bismuth Film Electrodes. Electroanalysis 15 (2003)1859-1863.

DOI: 10.1002/elan.200302763

[5] Benjamin J. Feldman , John D. Osterloh , Bernard H. Hata , Alessandra D'Alessandro (1994). Anal. Chem. 66 (13), p.1983–(1987).

[6] A.A.A. Khan, M.A. Abdullah. Bismuth-modified Hydroxyapatite Carbon Electrode for Simultaneous in-situ Cadmium and Lead Analysis Int. J. Electrochem. Sci. 8 (2013): 195-203.

[7] J.C. Quintana, et al.: Part I: A comparative study of bismuth-modified screen-printed electrodes for lead detection, Analyt. Chim. Acta, 707(1-2) (2011) 171-177.

DOI: 10.1016/j.aca.2011.08.052

[8] M.A. El Mhammedi, M. Achak, M. Bakasse, A. Chtaini, Physico-chemical characterization of electrochemical deposit of Ca10(PO4)6(OH)2 on copper surfaces, Appl. Surface Sci., 253 (14) (2007) 5925-5930.

DOI: 10.1016/j.apsusc.2007.01.085

[9] I. Mobasherpour, M.S. Heshajin, A. Kazemzadeh, M. Zakeri, Synthesis of nanocrystalline hydroxyapatite by using precipitation method', Journal of Alloys and Compounds, 430 (1-2) (2007) 330-333.

DOI: 10.1016/j.jallcom.2006.05.018

[10] M.N. Rasheed, M.M. Ahmed, A.F. Hossainy, S.M. Abd, Trends in speciation analysis of some heavy metals in serum of patients with chronic hepatitis C and chronic hepatitis B using differential pulse adsorptive spectrophotometry. J. Trace Elements in Med. Biol. 24 (2010).

DOI: 10.1016/j.jtemb.2009.11.006

[11] T.M. Majam, A.F. Al- Hossainy, U.A. Radwan Trace metal biomonitoring and assessment in ground water, soil and pearl millet (Penniseturn glaucum) plants in industrial and urban areas in Tehama by using square-wave voltammetry. Am-Eurasian J Agric Environ Sci, 5 (2009).