The Design and Simulated Analysis of Laser Beam-Shaping System for the Flow Cytometry

Abstract:

As for the requirement of size and energy distribution of focal spot in the laser detection area, this paper designs a laser beam-shaping system of flow cytometry. The 640nm red diode laser and 488nm blue solid laser which are arranged vertically are used as the excitation source. The emergent ray of two lasers is combined by beam splitter, and the combined beam is focused on the screen through two orthogonal cylindrical lens. The focal spot is compressed by doublet lens which is also used to eliminate chromatic aberration of focal spot. Applying ZEMAX software to optimize the whole optical system, the simulated results meet fully the actual experiment for individual blood cells, and it has certain theoretical significance for actual design of optical system about flow cytometry.

• Periodical: Applied Mechanics and Materials (Volumes 635-637)
• Pages: 1114-1117
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.635-637.1114
• Online since: September 2014
• Authors: Guo Dong Liu*, Xi Ying Hao, Zhen Huang, Zhong Ren
• Keywords: Excitation Source, Flow Cytometry, Laser Beam-Shaping System, Optical Design, ZEMAX Simulation

References

[1] S.T. Zhao: The study on test method and design for flow analyzer with single laser and four channel (University of Chinese Academy of Sciences press, china 2012).

[2] H.W. Zhi, W.J. Sun, J.N. Sun, and M.Y. Li: Optical Technology Vol. 38 (2012) no. 2, pp.236-239.

[3] H. Zhang, Y. Ji, and Z.X. Fan: Physical Experiment of College Vol. 20 (2007) no. 4, pp.23-24.

[4] Y.H. Gao, Z.Y. An, N.N. Li, W.X. Zhao, and J.S. Zhao: Optics and Precision Engineering Vol. 19 (2011) no. 7, pp.1464-1468.

[5] J.H. Ye, X.J. Hao, and H.C. Zhou: Engineering and Test Vol. 50 (2010) no. 3, pp.46-48.